Linked Life Data

1126576

Source:http://linkedlifedata.com/resource/pubmed/id/1126576

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1975-7-31
pubmed:abstractText
1. From enzyme-antienzyme kinetic studies a method for the determination of anti-n2-neuraminidase antibodies has been developed. This procedure is applicable in routine diagnosis. 2. Considering the present conditions, the reversibility of the virus neuraminidase antigen-antineuraminidase antibody complex has been proved. The inhibition must be described as a non-competitive one. 3. As a reference value for comparative immunity estimations the relative antibody concentration T150 is defined as the point of reciprocal serum dilution, where under a given substrate concentration the inhibited enzyme activity vi is half of the non-inhibited enzyme activity vo. 4. For the calculation of T150 a simple formula is derived from the Michaelis-Menten kinetic. Under present conditions the value of T150 is in a range of 1.5 log 2 dilution steps plus or minus the specific serum dilution T150 uninfluenced by possible variable parameters such as enzyme activity, substrate concentration and inhibitor concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
324-35
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Contribution to the standardization of influenza neuraminidase inhibition tests, based on enzyme- antienzyme kinetic studies.
pubmed:publicationType
Journal Article