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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2001-5-30
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pubmed:abstractText |
Gab-1 is a multiple docking protein that is tyrosine phosphorylated by receptor tyrosine kinases such as c-Met, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. We have now demonstrated that cell-cell adhesion also induces marked tyrosine phosphorylation of Gab-1 and that disruption of cell-cell adhesion results in its dephosphorylation. An anti-E-cadherin antibody decreased cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1, whereas the expression of E-cadherin specifically induced tyrosine phosphorylation of Gab-1. A relatively selective inhibitor of Src family kinases reduced cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1, whereas expression of a dominant-negative mutant of Csk increased it. Disruption of cell-cell adhesion, which reduced tyrosine phosphorylation of Gab-1, also reduced the activation of mitogen-activated protein kinase and Akt in response to cell-cell adhesion. These results indicate that E-cadherin-mediated cell-cell adhesion induces tyrosine phosphorylation by a Src family kinase of Gab-1, thereby regulating the activation of Ras/MAP kinase and phosphatidylinositol 3-kinase/Akt cascades.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18941-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11262408-Animals,
pubmed-meshheading:11262408-Cadherins,
pubmed-meshheading:11262408-Calcium,
pubmed-meshheading:11262408-Cell Adhesion,
pubmed-meshheading:11262408-Cell Line,
pubmed-meshheading:11262408-Enzyme Activation,
pubmed-meshheading:11262408-Genes, Dominant,
pubmed-meshheading:11262408-Glutathione Transferase,
pubmed-meshheading:11262408-Immunoblotting,
pubmed-meshheading:11262408-MAP Kinase Signaling System,
pubmed-meshheading:11262408-Mice,
pubmed-meshheading:11262408-Mutation,
pubmed-meshheading:11262408-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11262408-Phosphoproteins,
pubmed-meshheading:11262408-Phosphorylation,
pubmed-meshheading:11262408-Plasmids,
pubmed-meshheading:11262408-Precipitin Tests,
pubmed-meshheading:11262408-Protein Binding,
pubmed-meshheading:11262408-Recombinant Fusion Proteins,
pubmed-meshheading:11262408-Signal Transduction,
pubmed-meshheading:11262408-Tumor Cells, Cultured,
pubmed-meshheading:11262408-Tyrosine,
pubmed-meshheading:11262408-ras Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Roles of cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1.
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pubmed:affiliation |
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, 2-2 Yamada-oka, Suita 565-0871, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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