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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-3-22
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pubmed:abstractText |
The alymphoplasia (aly) mutation of mice prevents the development of systemic lymph nodes and Peyer's patches. The mutant homozygotes (aly/aly) are partially deficient in both humoral and cell-mediated immune functions. In the present study, we show that adult worm expulsion was slightly delayed and that T helper 2 (Th2)-type responses were partially defective in aly/aly mice after infection with Trichinella spiralis. Male aly/aly and aly/+ mice (8-weeks old) were infected with 400 muscle larvae. There was no difference in worm recovery between the two groups on day 5. However, worm recovery in aly/aly mice was significantly higher than that in aly/+ mice on day 14. Mucosal mast cells increased in number and peaked 14 days after infection in aly/+ mice. aly/aly mice were deficient in their mucosal mast cell response through out the primary infection. To examine the existence of mast cell precursors, aly/aly mice were treated with recombinant interleukin-3 (rIL-3) before infection. The mast cell response was poorly induced in aly/aly mice treated with rIL-3. An immunoglobulin E (IgE) response was not detected in aly/aly mice during the course of infection. Serum IgG1 levels in aly/aly mice were significantly lower than that of aly/+. The serum IgG2a levels increased in both strains of mice. However, IgG2a production in aly/aly mice on day 14 was half as much as that in aly/+mice. Stimulation of splenic T cells in vitro with anti-CD3 monoclonal antibody (mAb) showed that spleen cells from aly/+ mice on day 5 produced more IL-4 than spleen cells from aly/aly mice. IL-4 production from aly/aly mice on day 14 was half that from aly/+ mice. Interferon-gamma (IFN-gamma) was produced in both aly/aly and aly/+ mice on day 14. Proliferation assay showed that T cells of aly/aly mice responded poorly when cultured with antigen-presenting cells. These results suggest that aly gene is needed for the induction of protective immunity and Th2 responses in mice infected with T. spiralis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-10319865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-10510085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-10657657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-10799877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-1353100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-1836201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-1869831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-2142708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-2523861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-2831066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-2954900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-317031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-3876979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-4436751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-4571213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-6345400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-6386673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-6512640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-6979507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-7399701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-7636227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8171322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8299692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8671643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8675221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8691140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-8825203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9133428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-924522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9573108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9732696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9754555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9762559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11260327-9784657
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
218-24
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11260327-Animals,
pubmed-meshheading:11260327-Cell Culture Techniques,
pubmed-meshheading:11260327-Cell Division,
pubmed-meshheading:11260327-Immunity, Mucosal,
pubmed-meshheading:11260327-Immunoglobulin E,
pubmed-meshheading:11260327-Immunoglobulin G,
pubmed-meshheading:11260327-Immunologic Deficiency Syndromes,
pubmed-meshheading:11260327-Interferon-gamma,
pubmed-meshheading:11260327-Interleukin-4,
pubmed-meshheading:11260327-Intestinal Mucosa,
pubmed-meshheading:11260327-Male,
pubmed-meshheading:11260327-Mast Cells,
pubmed-meshheading:11260327-Mice,
pubmed-meshheading:11260327-Mice, Mutant Strains,
pubmed-meshheading:11260327-Point Mutation,
pubmed-meshheading:11260327-Spleen,
pubmed-meshheading:11260327-Th2 Cells,
pubmed-meshheading:11260327-Trichinella spiralis,
pubmed-meshheading:11260327-Trichinellosis
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pubmed:year |
2001
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pubmed:articleTitle |
Impaired protective immunity and T helper 2 responses in alymphoplasia (aly) mutant mice infected with Trichinella spiralis.
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pubmed:affiliation |
Department of Parasitology, Kochi Medical School, Nankoku City, Kochi, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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