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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2001-3-29
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pubmed:abstractText |
Omega--3 polyunsaturated fatty acids (PUFAs) are essential components required for normal cellular function and have been shown to exert many preventive and therapeutic actions. The amount of n--3 PUFAs is insufficient in most Western people, whereas the level of n--6 PUFAs is relatively too high, with an n--6/n--3 ratio of >18. These two classes of PUFAs are metabolically and functionally distinct and often have important opposing physiological functions; their balance is important for homeostasis and normal development. Elevating tissue concentrations of n--3 PUFAs in mammals relies on chronic dietary intake of fat rich in n--3 PUFAs, because mammalian cells lack enzymatic activities necessary either to synthesize the precursor of n--3 PUFAs or to convert n--6 to n--3 PUFAs. Here we report that adenovirus-mediated introduction of the Caenorhabditis elegans fat-1 gene encoding an n--3 fatty acid desaturase into mammalian cells can quickly and effectively elevate the cellular n--3 PUFA contents and dramatically balance the ratio of n--6/n--3 PUFAs. Heterologous expression of the fat-1 gene in rat cardiac myocytes rendered cells capable of converting various n--6 PUFAs to the corresponding n--3 PUFAs, and changed the n--6/n--3 ratio from about 15:1 to 1:1. In addition, an eicosanoid derived from n--6 PUFA (i.e., arachidonic acid) was reduced significantly in the transgenic cells. This study demonstrates an effective approach to modifying fatty acid composition of mammalian cells and also provides a basis for potential applications of this gene transfer in experimental and clinical settings.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10453342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10479232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10576293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10617967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10617994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10617995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10828169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10852422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-10901194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-1420300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-2931167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-3414486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-3459166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-3578100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-7295999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-8729114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-8847968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-8882978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9008460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9037020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9478278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9482916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9648502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9662755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259654-9792636
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4050-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11259654-Adenoviridae,
pubmed-meshheading:11259654-Animals,
pubmed-meshheading:11259654-Caenorhabditis elegans,
pubmed-meshheading:11259654-Cells, Cultured,
pubmed-meshheading:11259654-Eicosanoids,
pubmed-meshheading:11259654-Fatty Acid Desaturases,
pubmed-meshheading:11259654-Fatty Acids,
pubmed-meshheading:11259654-Gene Transfer Techniques,
pubmed-meshheading:11259654-Genetic Vectors,
pubmed-meshheading:11259654-Humans,
pubmed-meshheading:11259654-Myocardium,
pubmed-meshheading:11259654-Rats
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pubmed:year |
2001
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pubmed:articleTitle |
Adenoviral gene transfer of Caenorhabditis elegans n--3 fatty acid desaturase optimizes fatty acid composition in mammalian cells.
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pubmed:affiliation |
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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