|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2001-3-22
|
|
pubmed:abstractText |
Increased expression of the anti-apoptotic proteins Bcl-2 and Bcl-xL is involved in the development and progression of many tumors. We recently reported that the bcl-2/bcl-xL-bispecific antisense oligonucleotide 4625 induces apoptosis in lung carcinoma cells. To further assess the therapeutic potential of oligonucleotide 4625, we investigated its effect on a series of human tumor cell lines of diverse histologic origins in vitro and in vivo. Methods: Oligonucleotide 4625-mediated inhibition of bcl-2 and bcl-xL expression in vitro was measured in breast carcinoma cells with the use of reverse transcription-polymerase chain reaction (PCR), real-time PCR, and western blotting. Cytotoxicity was assessed in several different cell lines by measurement of tumor cell growth, propidium iodide uptake, and nuclear apoptosis. The in vivo activity of oligonucleotide 4625 was determined by the inhibition of growth of established tumor xenografts in nude mice, immunohistochemical staining of Bcl-2 and Bcl-x proteins in the tumors, and western blotting of tumor lysates. Apoptosis in tumor xenografts was detected with the use of in situ TUNEL (i.e., terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-digoxigenin nick end labeling) staining. All statistical tests are two-sided.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bcl2l1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Oligoribonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0027-8874
|
|
pubmed:author |
pubmed-author:BaumannBB,
pubmed-author:GautschiOO,
pubmed-author:HallJJ,
pubmed-author:LeechS HSH,
pubmed-author:OdermattBB,
pubmed-author:OlieR ARA,
pubmed-author:Simões-WüstA PAP,
pubmed-author:StahelR ARA,
pubmed-author:TschoppSS,
pubmed-author:Zangemeister-WittkeUU,
pubmed-author:ZieglerAA
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
21
|
|
pubmed:volume |
93
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
463-71
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11259472-Animals,
pubmed-meshheading:11259472-Antineoplastic Agents,
pubmed-meshheading:11259472-Apoptosis,
pubmed-meshheading:11259472-Blotting, Western,
pubmed-meshheading:11259472-Breast Neoplasms,
pubmed-meshheading:11259472-Carcinoma,
pubmed-meshheading:11259472-Colorectal Neoplasms,
pubmed-meshheading:11259472-Dose-Response Relationship, Drug,
pubmed-meshheading:11259472-Female,
pubmed-meshheading:11259472-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11259472-Humans,
pubmed-meshheading:11259472-Immunohistochemistry,
pubmed-meshheading:11259472-In Situ Nick-End Labeling,
pubmed-meshheading:11259472-Lung Neoplasms,
pubmed-meshheading:11259472-Male,
pubmed-meshheading:11259472-Melanoma,
pubmed-meshheading:11259472-Mice,
pubmed-meshheading:11259472-Mice, Nude,
pubmed-meshheading:11259472-Oligonucleotides,
pubmed-meshheading:11259472-Oligonucleotides, Antisense,
pubmed-meshheading:11259472-Oligoribonucleotides, Antisense,
pubmed-meshheading:11259472-Prostatic Neoplasms,
pubmed-meshheading:11259472-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11259472-RNA, Messenger,
pubmed-meshheading:11259472-RNA, Neoplasm,
pubmed-meshheading:11259472-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11259472-Skin Neoplasms,
pubmed-meshheading:11259472-Transplantation, Heterologous,
pubmed-meshheading:11259472-Tumor Cells, Cultured,
pubmed-meshheading:11259472-bcl-X Protein
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Activity of a novel bcl-2/bcl-xL-bispecific antisense oligonucleotide against tumors of diverse histologic origins.
|
|
pubmed:affiliation |
Division of Oncology, Department of Internal Medicine, University Hospital, Zurich, Switzerland.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
