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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0014597,
umls-concept:C0022646,
umls-concept:C0022702,
umls-concept:C0023089,
umls-concept:C0026018,
umls-concept:C0031164,
umls-concept:C0043047,
umls-concept:C0392747,
umls-concept:C0401925,
umls-concept:C0441633,
umls-concept:C0444706,
umls-concept:C0596292,
umls-concept:C0680730,
umls-concept:C1148554
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pubmed:issue |
4
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pubmed:dateCreated |
2001-3-22
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pubmed:abstractText |
The water channel aquaporin-2 (AQP2), a key component of the antidiuretic machinery in the kidney, is rapidly regulated by the antidiuretic hormone vasopressin. The hormone exerts its action by inducing a translocation of AQP2 from intracellular vesicles to the cell membrane. This step requires the elevation of intracellular cyclic AMP. We describe here a new method, laser scanning reflection microscopy (LSRM), suitable for determining cellular osmotic water permeability coefficient changes in primary cultured inner medullary collecting duct (IMCD) cells. The recording of vertical-reflection-mode x-z-scan section areas of unstained, living IMCD cells proved useful and valid for the investigation of osmotic water permeability changes. The time-dependent increases of reflection-mode x-z-scan section areas of swelling cells were fitted to a single-exponential equation. The analysis of the time constants of these processes indicates a twofold increase in osmotic water permeability of IMCD cells after treatment of the cells both with forskolin, a cyclic AMP-elevating agent, and with Clostridium difficile toxin B, an inhibitor of Rho proteins that leads to depolymerization of F-actin-containing stress fibers. This indicates that both agents lead to the functional insertion of AQP2 into the cell membrane. Thus, we have established a new functional assay for the study of the regulation of the water permeability at the cellular level.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-10620308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-10727991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-10867225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-10916423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-13065150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-1373524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-1745609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-5923067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-6951416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-7313695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-7540430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-7543677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-7971145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-8272373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-8798691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-9335376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-9438836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-9712891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-9815137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11259291-9988736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1783-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11259291-Actins,
pubmed-meshheading:11259291-Animals,
pubmed-meshheading:11259291-Bacterial Proteins,
pubmed-meshheading:11259291-Bacterial Toxins,
pubmed-meshheading:11259291-Cell Line,
pubmed-meshheading:11259291-Cyclic AMP,
pubmed-meshheading:11259291-Dogs,
pubmed-meshheading:11259291-Forskolin,
pubmed-meshheading:11259291-Kidney,
pubmed-meshheading:11259291-Kinetics,
pubmed-meshheading:11259291-Microscopy, Confocal,
pubmed-meshheading:11259291-Microscopy, Fluorescence,
pubmed-meshheading:11259291-Osmosis,
pubmed-meshheading:11259291-Protein Structure, Tertiary,
pubmed-meshheading:11259291-Protein Transport,
pubmed-meshheading:11259291-Rats,
pubmed-meshheading:11259291-Rats, Wistar,
pubmed-meshheading:11259291-Time Factors,
pubmed-meshheading:11259291-Water
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pubmed:year |
2001
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pubmed:articleTitle |
Cell volume kinetics of adherent epithelial cells measured by laser scanning reflection microscopy: determination of water permeability changes of renal principal cells.
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pubmed:affiliation |
Forschungsinstitut für Molekulare Pharmakologie, D-10315 Berlin, Germany. maric@fmp-berlin.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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