Linked Life Data

1125321

Source:http://linkedlifedata.com/resource/pubmed/id/1125321

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1975-8-4
pubmed:abstractText
The nature of the precursor pool for protein synthesis in porcine lymphocytes has been investigated. Intracellular free glycine was found to be used in preference to its extracellular counterpart. It is suggested that a similar preference applies for all amino acids. However, with certain amino acids such as phenylalanine, this preference is difficult to demonstrate because of a rapid exchange occurring between intracellular and extracellular pools. A small portion of the intracellular phenylalanine pool was found not to exchange rapidly but this was not important in the maintenance of protein synthesis. A different type of compartmentation of the intracellular glycine pool was apparent, but this was less well defined. During the course of these investigations it was demonstrated that the nature of the incubation medium is an important consideration for enabling correct interpretation of kinetic experiments. To maintain what appeared to be a constant rate of protein synthesis, lymphocytes had to be incubated in a comprehensive culture medium (basal Eagle's medium without serum); a declining rate of synthesis was observed if a simple buffered salts medium (Krebs-Ringer bicarbonate buffer) was used.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
390
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
363-73
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Incorporation of amino acids into protein from an intracellular pool of lymphocytes.
pubmed:publicationType
Journal Article