Linked Life Data

11251976

Source:http://linkedlifedata.com/resource/pubmed/id/11251976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-3-19
pubmed:abstractText
A unique lymphocyte lineage, the Valpha14 NKT cells, expresses both NK1.1 and an invariant antigen receptor encoded by Valpha14 and Jalpha281 gene segments. Valpha14 NKT cells play crucial roles in various immune responses, including autoimmune diseases, allergic reactions and anti-tumor immunity. Valpha14 NKT cells were demonstrated to be essential for anti-tumor effect of IL-12 in vivo. Here, we report that adoptive transfer of IL-12-activated Valpha14 NKT cells prevents hepatic metastasis of B16 melanoma. The injection of large amounts of IL-2, IL-4, and IFN-gamma, which are cytokines produced by activated Valpha14 NKT cells, exhibited no significant inhibition of the metastasis of this melanoma. The cells prepared from the liver of IL-12-injected mice expressed a potent cytotoxic activity on B16 melanoma cells in vitro. Although the adoptive transfer of IL-12-activated Valpha14 NKT cells prevents hepatic metastasis of B16 melanoma, activated NK cells from IL-12-injected RAG-1-/- mice failed to inhibit the metastasis of this melanoma. Thus, the anti-tumor effect of IL-12 can be replaced by adoptive transfer of IL-12-activated Valpha14 NKT cells but not by IL-12-activated NK cells, suggesting a minor role of NK cells for the IL-12-mediated anti-tumor effect in this experimental system. Moreover, our studies have suggested the involvement of direct cytotoxic mechanisms rather than cytokine-mediated immune responses at the effector phase of the Valpha14 NKT cell-mediated anti-tumor activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
523-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11251976-Adoptive Transfer, pubmed-meshheading:11251976-Animals, pubmed-meshheading:11251976-Cell Line, pubmed-meshheading:11251976-Chromium Radioisotopes, pubmed-meshheading:11251976-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11251976-Interferon-gamma, pubmed-meshheading:11251976-Interleukin-12, pubmed-meshheading:11251976-Interleukin-2, pubmed-meshheading:11251976-Interleukin-4, pubmed-meshheading:11251976-Killer Cells, Natural, pubmed-meshheading:11251976-Liver, pubmed-meshheading:11251976-Liver Neoplasms, pubmed-meshheading:11251976-Lymphocytes, pubmed-meshheading:11251976-Melanoma, Experimental, pubmed-meshheading:11251976-Mice, pubmed-meshheading:11251976-Mice, Transgenic, pubmed-meshheading:11251976-Neoplasm Metastasis, pubmed-meshheading:11251976-Neoplasm Transplantation, pubmed-meshheading:11251976-Neoplasms, pubmed-meshheading:11251976-Recombinant Proteins, pubmed-meshheading:11251976-Spleen, pubmed-meshheading:11251976-Time Factors
pubmed:year
2001
pubmed:articleTitle
Inhibition of tumor metastasis by adoptive transfer of IL-12-activated Valpha14 NKT cells.
pubmed:affiliation
CREST (Core Research for Evolutional Science and Technology) Project, Japan Science and Technology Corporation (JST), Department of Molecular Immunology, Graduate School of Medicine, Chiba University 1-8-1 Inohana, Chuo-ku, Chiba, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't