Source:http://linkedlifedata.com/resource/pubmed/id/11251861
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2001-3-29
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pubmed:abstractText |
The decreased incidence of graft-vs.-host disease found following umbilical cord blood (CB) transplantation, and the increased susceptibility of newborns to infections, have been attributed, in part, to functional and phenotypic immaturity of neonatal T cells. We investigated the phenotypic changes of CB T cells induced by two immunoregulary cytokines, interleukin (IL)-12 and IL-15, alone or in combination. Adult peripheral blood (APB) mononuclear cells (MNCs) were also tested for comparison. Prior to culture, the percentages of CD3+ CD8+, CD3+ CD25+, and CD3+ CD56+ cells were significantly lower in CB MNCs than in APB MNCs. IL-15, but not IL-12, significantly increased CD3+ CD8+ expression among the CB MNCs after 1 week of culture. Combining IL-12 and IL-15, however, resulted in decreased CB CD3+ CD8+ expression compared with IL-15 alone. The percentage of CD3+ CD25+ cells in CB MNCs spontaneously increased in the absence of cytokines, while that of CD3+ CD56+ cells in CB MNCs could not be enhanced with cytokines. In contrast, the percentages of CD3+ CD25+ and CD3+ CD56+ cells among the APB MNCs could be increased with IL-12, IL-15, and further with IL-12 and IL-15 combined. Thus, different patterns of T-cell subset changes were demonstrated between CB MNCs and APB MNCs in response to IL-12 and/or IL-15. These data may serve as a foundation for using cytokine therapy in newborns and children receiving CB transplants.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0905-6157
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11251861-Adult,
pubmed-meshheading:11251861-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11251861-CD8-Positive T-Lymphocytes,
pubmed-meshheading:11251861-Cell Survival,
pubmed-meshheading:11251861-Fetal Blood,
pubmed-meshheading:11251861-Flow Cytometry,
pubmed-meshheading:11251861-Humans,
pubmed-meshheading:11251861-Infant, Newborn,
pubmed-meshheading:11251861-Interleukin-12,
pubmed-meshheading:11251861-Interleukin-15,
pubmed-meshheading:11251861-Killer Cells, Natural,
pubmed-meshheading:11251861-Leukocytes, Mononuclear,
pubmed-meshheading:11251861-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:11251861-Phenotype,
pubmed-meshheading:11251861-T-Lymphocyte Subsets
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pubmed:year |
2001
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pubmed:articleTitle |
Phenotypic changes of T-lymphocyte subsets induced by interleukin-12 and interleukin-15 in umbilical cord vs. adult peripheral blood mononuclear cells.
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pubmed:affiliation |
Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung University, Taoyuan, Taiwan. sjlino@adm.cgmh.com.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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