Linked Life Data

11251853

Source:http://linkedlifedata.com/resource/pubmed/id/11251853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-3-19
pubmed:abstractText
The INO2 gene encodes a transcriptional activator of the phospholipid biosynthetic genes of Saccharomyces cerevisiae. Complete derepression of phospholipid biosynthetic gene expression in response to inositol/choline deprivation requires both INO2 and INO4. Ino2p dimerizes with Ino4p to bind the upstream activating sequence (UAS)INO element found in the promoters of the target genes. We have demonstrated previously that transcription from the INO2 promoter is autoregulated 12-fold in a manner identical to that of the target genes. Here, we show that this regulation occurs at the levels of transcription and translation. Transcription accounts for fourfold regulation, whereas translation accounts for an additional threefold regulation. Regulation of transcription requires a UAS(INO) element. Additional promoter elements include an upstream essential sequence (UES) located upstream of the UAS(INO) element and a negative regulatory element in the vicinity of the UAS(INO) element. Regulation of translation is dependent on an upstream open reading frame (uORF) in the INO2 leader. These data support the model that regulatory gene promoters may display unusual organizations and may be subject to multiple levels of regulation. We have shown previously that the UME6 gene positively regulates INO2 expression. Here, we limit the UME6-responsive region of the INO2 promoter to nucleotides -217 to -56.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1395-405
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11251853-Amino Acid Sequence, pubmed-meshheading:11251853-Base Sequence, pubmed-meshheading:11251853-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:11251853-Choline, pubmed-meshheading:11251853-Codon, pubmed-meshheading:11251853-DNA-Binding Proteins, pubmed-meshheading:11251853-Fungal Proteins, pubmed-meshheading:11251853-Gene Expression Regulation, Fungal, pubmed-meshheading:11251853-Genes, Fungal, pubmed-meshheading:11251853-Genes, Regulator, pubmed-meshheading:11251853-Inositol, pubmed-meshheading:11251853-Molecular Sequence Data, pubmed-meshheading:11251853-Mutation, pubmed-meshheading:11251853-Open Reading Frames, pubmed-meshheading:11251853-Promoter Regions, Genetic, pubmed-meshheading:11251853-Protein Biosynthesis, pubmed-meshheading:11251853-Repressor Proteins, pubmed-meshheading:11251853-Saccharomyces cerevisiae, pubmed-meshheading:11251853-Saccharomyces cerevisiae Proteins, pubmed-meshheading:11251853-Sequence Analysis, DNA, pubmed-meshheading:11251853-Transcription, Genetic, pubmed-meshheading:11251853-Transcription Factors
pubmed:year
2001
pubmed:articleTitle
Expression of the INO2 regulatory gene of Saccharomyces cerevisiae is controlled by positive and negative promoter elements and an upstream open reading frame.
pubmed:affiliation
Program in Molecular Biology, and Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, IL 60153, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't