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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-3-19
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pubmed:abstractText |
A crucial step in transcription is the recruitment of RNA polymerase to promoters. In the transcription of human rRNA genes by RNA Polymerase I (Pol I), transcription factor SL1 has a role as the essential core promoter binding factor. Little is known about the mechanism by which Pol I is recruited. We provide evidence for an essential role for hRRN3, the human homologue of a yeast Pol I transcription factor, in this process. We find that whereas the bulk of human Pol I complexes (I alpha) are transcriptionally inactive, hRRN3 defines a distinct subpopulation of Pol I complexes (I beta) that supports specific initiation of transcription. Human RRN3 interacts directly with TAF(I)110 and TAF(I)63 of promoter-selectivity factor SL1. Blocking this connection prevents recruitment of Pol I beta to the rDNA promoter. Furthermore, hRRN3 can be found in transcriptionally autonomous Pol I holoenzyme complexes. We conclude that hRRN3 functions to recruit initiation-competent Pol I to rRNA gene promoters. The essential role for hRRN3 in linking Pol I to SL1 suggests a mechanism for growth control of Pol I transcription.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-1009958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-10384269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-10384276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-10384303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-10471742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-10758157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-11032814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-11265758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-1495986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-1547496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-1638635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-2330041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-2390974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-3413483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-3621348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-3708692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-3768960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-3929071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-4070001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-4368415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-4472266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-7153690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-7491500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-7801123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-7801130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8007994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8306961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8413268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8641287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8670901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-8946909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9342329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9422795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9451438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9649439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9837969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11250903-9858602
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Holoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Pol1 Transcription Initiation...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase I,
http://linkedlifedata.com/resource/pubmed/chemical/RRN3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/transcription factor UBF,
http://linkedlifedata.com/resource/pubmed/chemical/transcription initiation factor...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1373-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11250903-Binding Sites,
pubmed-meshheading:11250903-Cell Nucleolus,
pubmed-meshheading:11250903-DNA-Binding Proteins,
pubmed-meshheading:11250903-Gene Expression Regulation,
pubmed-meshheading:11250903-Holoenzymes,
pubmed-meshheading:11250903-Humans,
pubmed-meshheading:11250903-Models, Genetic,
pubmed-meshheading:11250903-Pol1 Transcription Initiation Complex Proteins,
pubmed-meshheading:11250903-Promoter Regions, Genetic,
pubmed-meshheading:11250903-Protein Binding,
pubmed-meshheading:11250903-RNA, Ribosomal,
pubmed-meshheading:11250903-RNA Polymerase I,
pubmed-meshheading:11250903-Transcription, Genetic,
pubmed-meshheading:11250903-Transcription Factors
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pubmed:year |
2001
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pubmed:articleTitle |
hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters.
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pubmed:affiliation |
Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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