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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010749,
umls-concept:C0025914,
umls-concept:C0026237,
umls-concept:C0026809,
umls-concept:C0030685,
umls-concept:C0070971,
umls-concept:C0162638,
umls-concept:C0205231,
umls-concept:C0205421,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C1274040,
umls-concept:C1283071,
umls-concept:C1963578
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pubmed:issue |
1
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pubmed:dateCreated |
2001-3-15
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pubmed:abstractText |
Photodynamic therapy (PDT) activates the mitochondrial pathway of apoptosis, for which the release of cytochrome c into the cytosol is considered critical. To further elucidate the role of cytochrome c release in PDT-induced apoptosis, we monitored cytochrome c localization immunocytochemically and related it to nuclear apoptosis of the same cells. When mouse L5178Y-R cells were treated with 300 nM phthalocyanine (Pc) 4 and 0-75 mJ/cm(2) red light, cytochrome c release had a dose response similar to that of clonogenic cell killing, with nearly identical threshold doses. Within individual cells, the release of cytochrome c appeared to be an all-or-none phenomenon. Moreover, it was tightly associated with activation of a caspase-3-like protease and changes in nuclear morphology. Thus, in response to Pc 4-PDT, the release of cytochrome c from mitochondria is a key determinant of apoptotic cell death.
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pubmed:grant |
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pubmed:keyword |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
51-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11248418-Animals,
pubmed-meshheading:11248418-Apoptosis,
pubmed-meshheading:11248418-Caspase 3,
pubmed-meshheading:11248418-Caspases,
pubmed-meshheading:11248418-Cell Survival,
pubmed-meshheading:11248418-Cytochrome c Group,
pubmed-meshheading:11248418-Dose-Response Relationship, Drug,
pubmed-meshheading:11248418-Enzyme Activation,
pubmed-meshheading:11248418-Immunohistochemistry,
pubmed-meshheading:11248418-Indoles,
pubmed-meshheading:11248418-Leukemia L5178,
pubmed-meshheading:11248418-Mice,
pubmed-meshheading:11248418-Photochemotherapy,
pubmed-meshheading:11248418-Photosensitizing Agents,
pubmed-meshheading:11248418-Subcellular Fractions,
pubmed-meshheading:11248418-Time Factors,
pubmed-meshheading:11248418-Tumor Cells, Cultured,
pubmed-meshheading:11248418-Tumor Stem Cell Assay
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pubmed:year |
2001
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pubmed:articleTitle |
Phthalocyanine 4 photodynamic therapy-induced apoptosis of mouse L5178Y-R cells results from a delayed but extensive release of cytochrome c from mitochondria.
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pubmed:affiliation |
Department of Radiation Oncology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106-4942, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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