|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2001-3-14
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|
pubmed:abstractText |
High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.
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|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
1398-9219
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
2
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
99-104
|
|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:11247308-Amino Acid Sequence,
pubmed-meshheading:11247308-Animals,
pubmed-meshheading:11247308-Bacteria,
pubmed-meshheading:11247308-Base Sequence,
pubmed-meshheading:11247308-Gene Expression,
pubmed-meshheading:11247308-Genetic Techniques,
pubmed-meshheading:11247308-Genetic Vectors,
pubmed-meshheading:11247308-Immunoblotting,
pubmed-meshheading:11247308-Membrane Proteins,
pubmed-meshheading:11247308-Molecular Sequence Data,
pubmed-meshheading:11247308-R-SNARE Proteins,
pubmed-meshheading:11247308-Recombinant Fusion Proteins,
pubmed-meshheading:11247308-SNARE Proteins,
pubmed-meshheading:11247308-Vesicular Transport Proteins
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Strategies for prokaryotic expression of eukaryotic membrane proteins.
|
|
pubmed:affiliation |
Universität Heidelberg, Neurobiology Department, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
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|
pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
|
