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rdf:type |
|
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-3-14
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|
pubmed:abstractText |
The new fluorescence marker Sulforodamine 101 that allows one to avoid a labour-consuming stage of purification of a marker substance (for example, calceine, other fluorescein derivatives) from hydrophobic admixtures that reduce the storage of a liposomal reagent was used in liposomal immunoassay. Sulforodamine 101 shows the maximum increase in a fluorescence signal when liposomes are destroyed with encapsulated marker, which is 45 times greater than does Sulforodamine B (8 times) and comparable with calceine (30 times). The comparative studies using liposomes containing calceine and Sulforodamine 101 have indicated that they are similar storage and sensitivity (50 ng/ml) of liposomal test system as determined by the concentration of F. tularensis polysaccharides in solutions.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0869-6047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-24
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
[Sulforodamine 101: novel effective marker in liposomal immunoassay].
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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