Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-3-14
pubmed:abstractText
Insulin stimulates K+ transport by the Na+-K+-ATPase in human fibroblasts. In other cell systems, this action represents an automatic response to increased intracellular [Na+] or results from translocation of transporters from an intracellular site to the plasma membrane. Here we evaluate whether these mechanisms are operative in human fibroblasts. Human fibroblasts expressed the alpha(1) but not the alpha(2) and alpha(3) isoforms of Na+-K+-ATPase . Insulin increased the influx of Rb+, used to trace K+ entry, but did not modify the total intracellular content of K+, Rb+, and Na+ over a 3-h incubation period. Ouabain increased intracellular Na+ more rapidly in cells incubated with insulin, but this increase followed insulin stimulation of Rb+ transport. Bumetanide did not prevent the increased Na+ influx or stimulation of Na+-K+-ATPase. Stimulation of the Na+-K+-ATPase by insulin did not produce any measurable change in membrane potential. Insulin did not affect the affinity of the pump toward internal Na+ or the number of membrane-bound Na+-K+-ATPases, as assessed by ouabain binding. By contrast, insulin slightly increased the affinity of Na+-K+-ATPase toward ouabain. Phorbol esters did not mimic insulin action on Na+-K+-ATPase and inhibited, rather than stimulated, Rb+ transport. These results indicate that insulin increases the turnover rate of Na+-K+-ATPases of human fibroblasts without affecting their number on the plasma membrane or modifying their dependence on intracellular [Na+].
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes, http://linkedlifedata.com/resource/pubmed/chemical/Bumetanide, http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens, http://linkedlifedata.com/resource/pubmed/chemical/Diuretics, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hypoglycemic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Ouabain, http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Rubidium, http://linkedlifedata.com/resource/pubmed/chemical/Sodium, http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase, http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C912-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11245608-Androstadienes, pubmed-meshheading:11245608-Biological Transport, pubmed-meshheading:11245608-Blotting, Northern, pubmed-meshheading:11245608-Bumetanide, pubmed-meshheading:11245608-Carcinogens, pubmed-meshheading:11245608-Cell Membrane, pubmed-meshheading:11245608-Cells, Cultured, pubmed-meshheading:11245608-Diuretics, pubmed-meshheading:11245608-Enzyme Inhibitors, pubmed-meshheading:11245608-Fibroblasts, pubmed-meshheading:11245608-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11245608-Humans, pubmed-meshheading:11245608-Hypoglycemic Agents, pubmed-meshheading:11245608-Insulin, pubmed-meshheading:11245608-Membrane Potentials, pubmed-meshheading:11245608-Ouabain, pubmed-meshheading:11245608-Phorbol 12,13-Dibutyrate, pubmed-meshheading:11245608-RNA, Messenger, pubmed-meshheading:11245608-Rubidium, pubmed-meshheading:11245608-Sodium, pubmed-meshheading:11245608-Sodium-Potassium-Exchanging ATPase
pubmed:year
2001
pubmed:articleTitle
Insulin increases the turnover rate of Na+-K+-ATPase in human fibroblasts.
pubmed:affiliation
Division of Medical Genetics, Department of Pediatrics, Emory University, 2040 Ridgewood Drive, Atlanta, GA 30322, USA. nl@rw.ped.emory.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.