Linked Life Data

11245572

Source:http://linkedlifedata.com/resource/pubmed/id/11245572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-3-14
pubmed:abstractText
In remarkable contrast to somatic cells, the germline of the nematode Caenorhabditis elegans efficiently silences transgenic DNA. The molecular mechanisms responsible for this have been shown to implicate chromatin proteins encoded by the mes genes (Kelly, W. G. and Fire, A. (1998) Development 125, 2451-2456), of which two are the C. elegans homologs of Polycomb Group gene transcriptional repressors. We have analyzed the contribution of the histone H1 gene family to this specific aspect of germ cells in C. elegans. We show with isotype-specific double stranded RNA-mediated interference (RNAi) that a single member of this gene family (H1.1) is essential for the repression of a silenced reporter-transgene in the germline of hermaphrodites and males, whereas no change is found in the somatic expression of this reporter. Additionally, RNA-mediated interference with H1.1 gene expression can cause a phenotype with severe affection of germline proliferation and differentiation in the hermaphrodite, and even sterility (5%-11% penetrance). These and further features observed in histone H1.1 RNAi experiments are also characteristic of the mes phenotype (Garvin, C., Holdeman, R. and Strome, S. (1998) Genetics 148, 167-185), which is believed to result from the desilencing of genes required for somatic differentiation in the germline. Our observations therefore support this interpretation of the mes phenotype and they identify a single histone H1 isoform (H1.1) as a new component specifically involved in chromatin silencing in the germline of C. elegans.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
A single histone H1 isoform (H1.1) is essential for chromatin silencing and germline development in Caenorhabditis elegans.
pubmed:affiliation
Third Department of Zoology--Developmental Biology, University of Göttingen, Humboldtalle 34A, D-37073 Göttingen, Germany. mjedrus@gwdg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't