Source:http://linkedlifedata.com/resource/pubmed/id/11243834
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-3-13
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pubmed:abstractText |
The chemical synthesis of RNA oligonucleotides is a valuable resource for biological research. A new approach for RNA synthesis that is now as reliable and efficient as DNA synthesis methods is described in this report. A 5'-O-silyl ether is used in conjunction with acid-labile orthoester protecting groups on the 2'-hydroxyls. RNA synthesis proceeds efficiently on commercial synthesizers in high yields. Analysis by anion-exchange HPLC shows that the quality and yields of RNA synthesized with this chemistry are unprecedented. Furthermore, this chemistry enables analysis and purification of stable 2'-O-protected RNA. This property serves to minimize possibilities for degradation of the RNA. In addition, it now possible to analyze troublesome sequences, which, when fully 2'-O-deprotected, do not easily resolve into one major conformation due to strong secondary structure. When ready for use, the RNA is easily 2'-O-deprotected in mild-acidic aqueous buffers in 30 min. This new RNA chemistry has enabled the routine high-quality synthesis of RNA oligonucleotides up to 50 bases in length regardless of sequence or secondary structure.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Organosilicon Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/RNA
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1046-2023
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
206-17
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pubmed:meshHeading |
pubmed-meshheading:11243834-Anions,
pubmed-meshheading:11243834-Chromatography, High Pressure Liquid,
pubmed-meshheading:11243834-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11243834-Esters,
pubmed-meshheading:11243834-Genetic Techniques,
pubmed-meshheading:11243834-Models, Chemical,
pubmed-meshheading:11243834-Oligonucleotides,
pubmed-meshheading:11243834-Organosilicon Compounds,
pubmed-meshheading:11243834-RNA,
pubmed-meshheading:11243834-Time Factors
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pubmed:year |
2001
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pubmed:articleTitle |
RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry.
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pubmed:affiliation |
Dharmacon Research, Inc, 1376 Miners Drive, Lafayette, Colorado 80026, USA. scaringe@dharmacon.com
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pubmed:publicationType |
Journal Article
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