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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6824
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pubmed:dateCreated |
2001-3-12
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pubmed:abstractText |
In all synapses, Ca2+ triggers neurotransmitter release to initiate signal transmission. Ca2+ presumably acts by activating synaptic Ca2+ sensors, but the nature of these sensors--which are the gatekeepers to neurotransmission--remains unclear. One of the candidate Ca2+ sensors in release is the synaptic Ca2+-binding protein synaptotagmin I. Here we have studied a point mutation in synaptotagmin I that causes a twofold decrease in overall Ca2+ affinity without inducing structural or conformational changes. When introduced by homologous recombination into the endogenous synaptotagmin I gene in mice, this point mutation decreases the Ca2+ sensitivity of neurotransmitter release twofold, but does not alter spontaneous release or the size of the readily releasable pool of neurotransmitters. Therefore, Ca2+ binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
410
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11242035-Animals,
pubmed-meshheading:11242035-Calcium,
pubmed-meshheading:11242035-Calcium-Binding Proteins,
pubmed-meshheading:11242035-Cells, Cultured,
pubmed-meshheading:11242035-Membrane Glycoproteins,
pubmed-meshheading:11242035-Mice,
pubmed-meshheading:11242035-Mutagenesis, Site-Directed,
pubmed-meshheading:11242035-Nerve Tissue Proteins,
pubmed-meshheading:11242035-Neurons,
pubmed-meshheading:11242035-Neurotransmitter Agents,
pubmed-meshheading:11242035-Point Mutation,
pubmed-meshheading:11242035-Protein Binding,
pubmed-meshheading:11242035-Protein Conformation,
pubmed-meshheading:11242035-Synapses,
pubmed-meshheading:11242035-Synaptic Vesicles,
pubmed-meshheading:11242035-Synaptotagmin I,
pubmed-meshheading:11242035-Synaptotagmins
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pubmed:year |
2001
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pubmed:articleTitle |
Synaptotagmin I functions as a calcium regulator of release probability.
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pubmed:affiliation |
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas 75390-9111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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