Linked Life Data

11241316

Source:http://linkedlifedata.com/resource/pubmed/id/11241316

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-3-12
pubmed:abstractText
Predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. Evidence suggests that the lectin Helix pomatia agglutinin (HPA) identifies oligosaccharides associated with poor-prognosis cancer. Our aim was to identify oligosaccharides that bind HPA in aggressive breast cancers. Breast-cancer cell lines (MCF-7, BT-549 and BT-20) and a cell line from human milk (HBL-100), which showed a range of HPA-binding intensities, were used to extract HPA-binding glycoproteins. Oligosaccharides were released using anhydrous hydrazine and separated on a range of HPLC matrices. We investigated whether HPA-binding oligosaccharides from cell lines were present in human breast-cancer tissues, using 69 breast-cancer specimens from patients with between 5 and 10 years' follow-up. A monosialylated oligosaccharide was over-expressed in the cell line that bound HPA strongly. Further analysis by normal-phase HPLC showed that the 2-aminobenzamide-conjugated oligosaccharide had a hydrodynamic volume of 4.58 glucose units (HPAgly1). Increased expression of HPAgly1 was associated with HPA staining of breast-cancer specimens (Student's t-test p = 0.025). Analysis of oligosaccharide levels and disease-free survival after treatment for breast cancer indicated a shorter disease-free interval for patients with elevated levels of HPAgly1. This is the first time that histochemical lectin staining has been correlated with biochemical mapping of oligosaccharides. Using this approach, we have identified a monosialylated HPA lectin-binding oligosaccharide present in breast-cancer cells grown in vitro which is elevated in breast-cancer specimens that bind the lectin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
79-85
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed-meshheading:11241316-Adult, pubmed-meshheading:11241316-Anthranilic Acids, pubmed-meshheading:11241316-Breast Neoplasms, pubmed-meshheading:11241316-Carcinoma, Ductal, Breast, pubmed-meshheading:11241316-Cell Line, pubmed-meshheading:11241316-Chromatography, Affinity, pubmed-meshheading:11241316-Chromatography, High Pressure Liquid, pubmed-meshheading:11241316-Chromatography, Ion Exchange, pubmed-meshheading:11241316-Disease-Free Survival, pubmed-meshheading:11241316-Female, pubmed-meshheading:11241316-Fluorescent Dyes, pubmed-meshheading:11241316-Humans, pubmed-meshheading:11241316-Lectins, pubmed-meshheading:11241316-Middle Aged, pubmed-meshheading:11241316-Oligosaccharides, pubmed-meshheading:11241316-Prognosis, pubmed-meshheading:11241316-Protein Binding, pubmed-meshheading:11241316-Retrospective Studies, pubmed-meshheading:11241316-Time Factors, pubmed-meshheading:11241316-Treatment Outcome, pubmed-meshheading:11241316-Tumor Cells, Cultured
pubmed:year
2001
pubmed:articleTitle
Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer.
pubmed:affiliation
Department of Surgery, Royal Free and University College London Medical School, London, United Kingdom. m.dwek@ucl.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't