Linked Life Data

11241183

Source:http://linkedlifedata.com/resource/pubmed/id/11241183

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-3-12
pubmed:abstractText
In the rat pancreatic beta-cell, Na/Ca exchange displays a quite high capacity. The cell is equipped with two alternatively spliced Na/Ca exchanger-1 (NCX1) isoforms, namely NCX1.3 and NCX1.7. To examine the existence of a possible functional difference between these splice variants, they were cloned, together with the heart variant NCX1.1, and expressed in human embryonic kidney-293 (HEK293) and Chinese hamster ovary (CHO) cells. In these two systems, the three splice variants showed a comparable level of intracellular Na+ (Na+(i))-dependent extracellular Ca2+(Ca2+(o)) uptake. Different levels of NCX1.3 and NCX1.7 transcripts were found in four rodent species, together with a marked interspecies difference in Na/Ca exchange activity. Three additional splice variants were found (NCX1.2, NCX1.9 and NCX1.13) in guinea-pigs, hamsters and mice, again in different proportions. Our data provide evidence for the activity of the three NCX1 splice variants. They also show the existence of a differential and species-specific transcription pattern of NCX1 gene products in pancreatic islet cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
517-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
NCX1 Na/Ca exchanger splice variants in pancreatic islet cells.
pubmed:affiliation
Laboratory of Pharmacology, Brussels University School of Medicine, Bâtiment GE, 808 route de Lennik, B-1070 Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't