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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-3-12
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pubmed:abstractText |
Saccharomyces cells with one unrepaired double-strand break (DSB) adapt after checkpoint-mediated G2/M arrest. Adaptation is accompanied by loss of Rad53p checkpoint kinase activity and Chk1p phosphorylation. Rad53p kinase remains elevated in yku70delta and cdc5-ad cells that fail to adapt. Permanent G2/M arrest in cells with increased single-stranded DNA is suppressed by the rfa1-t11 mutation, but this RPA mutation does not suppress permanent arrest in cdc5-ad cells. Checkpoint kinase activation and inactivation can be followed in G2-arrested cells, but there is no kinase activation in G1-arrested cells. We conclude that activation of the checkpoint kinases in response to a single DNA break is cell cycle regulated and that adaptation is an active process by which these kinases are inactivated.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/CEF1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Checkpoint kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Ku autoantigen,
http://linkedlifedata.com/resource/pubmed/chemical/MEC1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RAD53 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Replication Protein A,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/XRCC5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/high affinity DNA-binding factor...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
293-300
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:11239458-Adaptation, Biological,
pubmed-meshheading:11239458-Anaphase,
pubmed-meshheading:11239458-Antigens, Nuclear,
pubmed-meshheading:11239458-Cell Cycle,
pubmed-meshheading:11239458-Cell Cycle Proteins,
pubmed-meshheading:11239458-DNA, Fungal,
pubmed-meshheading:11239458-DNA Damage,
pubmed-meshheading:11239458-DNA Helicases,
pubmed-meshheading:11239458-DNA-Binding Proteins,
pubmed-meshheading:11239458-Enzyme Activation,
pubmed-meshheading:11239458-Fungal Proteins,
pubmed-meshheading:11239458-G2 Phase,
pubmed-meshheading:11239458-Gene Expression,
pubmed-meshheading:11239458-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:11239458-Kinetics,
pubmed-meshheading:11239458-Mitosis,
pubmed-meshheading:11239458-Mutation,
pubmed-meshheading:11239458-Nuclear Proteins,
pubmed-meshheading:11239458-Phosphorylation,
pubmed-meshheading:11239458-Protein Kinases,
pubmed-meshheading:11239458-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11239458-RNA-Binding Proteins,
pubmed-meshheading:11239458-Recombination, Genetic,
pubmed-meshheading:11239458-Replication Protein A,
pubmed-meshheading:11239458-Saccharomyces cerevisiae,
pubmed-meshheading:11239458-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:11239458-Sequence Homology, Nucleic Acid
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pubmed:year |
2001
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pubmed:articleTitle |
Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest.
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pubmed:affiliation |
Istituto F.I.R.C. di Oncologia Molecolare and, Dipartimento di Genetica e di Biologia dei, Microrganismi, Universita' degli Studi di Milano, 20133, Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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