Linked Life Data

11238679

Source:http://linkedlifedata.com/resource/pubmed/id/11238679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-3-12
pubmed:abstractText
Dendritic cells (DC) represent potent APCs that are capable of generating tumor-specific immunity. We performed a pilot clinical trial using Ag-pulsed DC as a tumor vaccine. Twenty-one patients with metastatic prostate cancer received two monthly injections of DC enriched and activated from their PBMC. DC were cocultured ex vivo with recombinant mouse prostatic acid phosphatase as the target neoantigen. Following enrichment, DC developed an activated phenotype with up-regulation of CD80, CD86, and CD83 expression. During culture, the DC maintained their levels of various adhesion molecules, including CD44, LFA-1, cutaneous lymphocyte-associated Ag, and CD49d, up-regulated CCR7, but lost CD62 ligand and CCR5. In the absence of CD62 ligand, such cells would not be expected to prime T cells efficiently if administered i.v. due to their inability to access lymphoid tissue via high endothelial venules. To assess this possibility, three patient cohorts were immunized with Ag-pulsed DC by i.v., intradermal (i.d.), or intralymphatic (i.l.) injection. All patients developed Ag-specific T cell immune responses following immunization, regardless of route. Induction of IFN-gamma production, however, was seen only with i.d. and i.l. routes of administration, and no IL-4 responses were seen regardless of route, consistent with the induction of Th1-type immunity. Five of nine patients who were immunized by the i.v. route developed Ag-specific Abs compared with one of six for i.d. and two of six for i.l. routes. These results suggest that while activated DC can prime T cell immunity regardless of route, the quality of this response and induction of Ag-specific Abs may be affected by the route of administration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4254-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11238679-Acid Phosphatase, pubmed-meshheading:11238679-Antibody Specificity, pubmed-meshheading:11238679-Cancer Vaccines, pubmed-meshheading:11238679-Cell Adhesion Molecules, pubmed-meshheading:11238679-Cells, Cultured, pubmed-meshheading:11238679-Cytokines, pubmed-meshheading:11238679-Dendritic Cells, pubmed-meshheading:11238679-Flow Cytometry, pubmed-meshheading:11238679-Humans, pubmed-meshheading:11238679-Injections, Intradermal, pubmed-meshheading:11238679-Injections, Intralymphatic, pubmed-meshheading:11238679-Injections, Intravenous, pubmed-meshheading:11238679-Lymphocyte Activation, pubmed-meshheading:11238679-Male, pubmed-meshheading:11238679-Pilot Projects, pubmed-meshheading:11238679-Prostate, pubmed-meshheading:11238679-Prostatic Neoplasms, pubmed-meshheading:11238679-Receptors, Chemokine, pubmed-meshheading:11238679-Receptors, Lymphocyte Homing, pubmed-meshheading:11238679-T-Lymphocytes
pubmed:year
2001
pubmed:articleTitle
Dendritic cells injected via different routes induce immunity in cancer patients.
pubmed:affiliation
Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94304, USA. lfong@stanfrod.edu
pubmed:publicationType
Journal Article, Clinical Trial, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't