Source:http://linkedlifedata.com/resource/pubmed/id/11238678
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-3-12
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| pubmed:abstractText |
We investigated the effect of IL-10 on replication of primary CXCR4-dependent (X4) HIV-1 strains by monocyte-derived dendritic cells (DCs) and macrophages (M Phis). M Phis efficiently replicated CXCR4-dependent HIV-1 (X4 HIV-1) strains NDK and VN44, whereas low levels of p24 were detected in supernatants of infected DCs. IL-10 significantly increased X4 HIV-1 replication by DCs but blocked viral production by M Phis as determined by p24 levels and semiquantitative nested PCR. IL-10 up-regulated CXCR4 mRNA and protein expression on DCs and M Phis, suggesting that IL-10 enhances virus entry in DCs but blocks an entry and/or postentry step in M Phis. The effect of IL-10 on the ability of DCs and M Phis to transmit virus to autologous CD4(+) T lymphocytes was investigated in coculture experiments. DCs exhibited a greater ability than did M Phis to transmit a vigorous infection to CD4(+) T cells despite their very low replication capacity. IL-10 had no effect on HIV-1 replication in DC:T cell cocultures but markedly decreased viral production in M Phi:T cell cocultures. These results demonstrate that IL-10 has opposite effects on the replication of primary X4 HIV-1 strains by DCs and M Phis. IL-10 increases X4-HIV-1 replication in DCs but does not alter their capacity to transmit virus to CD4(+) T lymphocytes. These findings suggest that increased levels of IL-10 observed in HIV-1-infected patients with disease progression may favor the replication of X4 HIV-1 strains in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
166
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4244-53
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11238678-Antigens, CD4,
pubmed-meshheading:11238678-Antiviral Agents,
pubmed-meshheading:11238678-Cell Differentiation,
pubmed-meshheading:11238678-Cells, Cultured,
pubmed-meshheading:11238678-Chemokine CCL5,
pubmed-meshheading:11238678-Coculture Techniques,
pubmed-meshheading:11238678-DNA, Viral,
pubmed-meshheading:11238678-Dendritic Cells,
pubmed-meshheading:11238678-Down-Regulation,
pubmed-meshheading:11238678-Gene Dosage,
pubmed-meshheading:11238678-HIV-1,
pubmed-meshheading:11238678-Humans,
pubmed-meshheading:11238678-Interleukin-10,
pubmed-meshheading:11238678-Macrophages,
pubmed-meshheading:11238678-Monocytes,
pubmed-meshheading:11238678-Receptors, CXCR4,
pubmed-meshheading:11238678-T-Lymphocytes,
pubmed-meshheading:11238678-Up-Regulation,
pubmed-meshheading:11238678-Virus Replication
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Opposite effects of IL-10 on the ability of dendritic cells and macrophages to replicate primary CXCR4-dependent HIV-1 strains.
|
| pubmed:affiliation |
Unité d'Immunopathologie Humaine, Institut National de la Santé et de la Recherche Médicale, Broussais Hospital, Paris, France. ancapetronela@hotmail.com
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|