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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-3-12
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pubmed:abstractText |
C57BL/6 (B6) mice with targeted mutations of immune function genes were used to investigate the mechanism of recovery from experimental autoimmune encephalomyelitis (EAE). The acute phase of passive EAE in the B6 mouse is normally resolved by partial recovery followed by mild sporadic relapses. B6 TCR beta-chain knockout (KO) recipients of a myelin oligodendrocyte glycoprotein p35-55 encephalitogenic T cell line failed to recover from the acute phase of passive EAE. In comparison with wild-type mice, active disease was more severe in beta(2)-microglobulin KO mice. Reconstitution of TCR beta-chain KO mice with wild-type spleen cells halted progression of disease and favored recovery. Spleen cells from T cell-deficient mice, IL-7R KO mice, or IFN-gamma KO mice were ineffective in this regard. Irradiation or treatment of wild-type spleen cell population with anti-NK1.1 mAb before transfer abrogated the protective effect. Removal of DX5(+) cells from wild-type spleen cells by anti-DX5 Ab-coated magnetic beads before reconstitution abrogated the suppressive properties of the spleen cells. TCR-deficient recipients of the enriched DX5(+) cell population recovered normally from passively induced acute disease. DX5(+) cells were sorted by FACS into DX5(+) alpha beta TCR(+) and DX5(+) alpha beta TCR(-) populations. Only recipients of the former recovered normally from clinical disease. These results indicate that recovery from acute EAE is an active process that requires NK1.1(+), DX5(+) alpha beta(+) TCR spleen cells and IFN-gamma.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Klrb1c protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin-Associated Glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin,
http://linkedlifedata.com/resource/pubmed/chemical/myelin oligodendrocyte glycoprotein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
166
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4209-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11238673-Acute Disease,
pubmed-meshheading:11238673-Adoptive Transfer,
pubmed-meshheading:11238673-Amino Acid Sequence,
pubmed-meshheading:11238673-Animals,
pubmed-meshheading:11238673-Antigens,
pubmed-meshheading:11238673-Antigens, Ly,
pubmed-meshheading:11238673-Antigens, Surface,
pubmed-meshheading:11238673-Biological Markers,
pubmed-meshheading:11238673-Cell Line,
pubmed-meshheading:11238673-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:11238673-Flow Cytometry,
pubmed-meshheading:11238673-Interferon-gamma,
pubmed-meshheading:11238673-Killer Cells, Natural,
pubmed-meshheading:11238673-Lectins, C-Type,
pubmed-meshheading:11238673-Lymphocyte Depletion,
pubmed-meshheading:11238673-Lymphopenia,
pubmed-meshheading:11238673-Male,
pubmed-meshheading:11238673-Mice,
pubmed-meshheading:11238673-Mice, Inbred C57BL,
pubmed-meshheading:11238673-Mice, Knockout,
pubmed-meshheading:11238673-Mice, Transgenic,
pubmed-meshheading:11238673-Molecular Sequence Data,
pubmed-meshheading:11238673-Myelin Proteins,
pubmed-meshheading:11238673-Myelin-Associated Glycoprotein,
pubmed-meshheading:11238673-NK Cell Lectin-Like Receptor Subfamily B,
pubmed-meshheading:11238673-Peptide Fragments,
pubmed-meshheading:11238673-Protein Biosynthesis,
pubmed-meshheading:11238673-Proteins,
pubmed-meshheading:11238673-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:11238673-Spleen,
pubmed-meshheading:11238673-T-Lymphocyte Subsets,
pubmed-meshheading:11238673-beta 2-Microglobulin
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pubmed:year |
2001
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pubmed:articleTitle |
Regulation of experimental autoimmune encephalomyelitis in the C57BL/6J mouse by NK1.1+, DX5+, alpha beta+ T cells.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA. rfritz@mcw.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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