Linked Life Data

11238638

Source:http://linkedlifedata.com/resource/pubmed/id/11238638

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-3-12
pubmed:abstractText
The human leukocyte receptor complex (LRC) on chromosome 19q13.4 encodes Ig superfamily receptors expressed on hemopoietic cells. Killer Ig-like receptors (KIR) are expressed in cytotoxic lymphocytes but other LRC molecules (Ig-like transcript(ILT)/leukocyte Ig-like receptor (LIR)) are more ubiquitous. We investigated expression of the ILT2/LIR1 inhibitory receptor compared with the related KIR. Both ILT2/LIR1 and KIR were expressed by peripheral CD8(+) T cells with a memory/effector phenotype. ILT2/LIR1(+) T cells demonstrated diverse TCRBV repertoires in contrast to KIR(+) T cells, while numbers of peripheral ILT2/LIR1(+) T cells were greater than KIR(+) T cells and the majority of ILT2/LIR1(+) T cells did not coexpress KIR. Analysis of CD8(+) T cells with specific HLA class I tetramers confirmed this pattern of expression, indicating differential regulation of LRC gene expression in T lymphocytes. Only a minor proportion of ILT2/LIR1(+) KIR(-) clones survived in vitro cloning, were more susceptible to anti-CD3 or cognate peptide induced cell death than KIR(+) T cells and exhibited lower levels of the Bcl-2 survival molecule. Our results indicate a sequential program of LRC-encoded receptor expression with initial ILT2/LIR1 expression in effector T cells and KIR gene transcription in the minor proportion of expanded clones which survives activation-induced cell death to become long term memory T cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3933-41
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11238638-Antigens, CD, pubmed-meshheading:11238638-Cell Death, pubmed-meshheading:11238638-Cell Line, Transformed, pubmed-meshheading:11238638-Clone Cells, pubmed-meshheading:11238638-Flow Cytometry, pubmed-meshheading:11238638-Humans, pubmed-meshheading:11238638-Immunoglobulins, pubmed-meshheading:11238638-Immunologic Memory, pubmed-meshheading:11238638-Lymphocyte Activation, pubmed-meshheading:11238638-Lymphocyte Subsets, pubmed-meshheading:11238638-Multigene Family, pubmed-meshheading:11238638-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:11238638-Receptors, Immunologic, pubmed-meshheading:11238638-Receptors, KIR, pubmed-meshheading:11238638-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11238638-T-Lymphocyte Subsets, pubmed-meshheading:11238638-T-Lymphocytes, Cytotoxic
pubmed:year
2001
pubmed:articleTitle
Differential expression of leukocyte receptor complex-encoded Ig-like receptors correlates with the transition from effector to memory CTL.
pubmed:affiliation
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. neil.young@nds.ox.ac.uk
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't