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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-3-12
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pubmed:databankReference |
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pubmed:abstractText |
The nociceptin receptor (Noci-R) is a G protein-coupled receptor present in neural tissues and its activation by nociceptin is involved in the processing of pain signals. Here, we report that Noci-R is present and functional on peripheral blood polymorphonuclear leukocytes (PMN). Human PMN express mRNA for Noci-R, its nucleotide sequence determined, and specific binding with [(125)I]-labeled nociceptin gave an apparent K(d) approximately 1.5 nM for this PMN opioid receptor. Nociceptin evoked PMN chemotaxis with maximal activity at 100 pM, without intracellular Ca(2+) mobilization. When injected in murine air pouches, nociceptin elicited leukocyte infiltration in a concentration-dependent fashion. Nociceptin-stimulated PMN infiltration was inhibited by treating mice with a synthetic analog of the aspirin-triggered lipid mediator 15-epi-lipoxin A(4). The present results identify nociceptin as a potent chemoattractant and provide a novel link between the neural and immune systems that are blocked by aspirin-triggered lipid mediators and may be relevant in neurogenic inflammation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Aspirin,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/lipoxin A4,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
166
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3650-4
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11238602-Animals,
pubmed-meshheading:11238602-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:11238602-Aspirin,
pubmed-meshheading:11238602-Calcium Signaling,
pubmed-meshheading:11238602-Cell Movement,
pubmed-meshheading:11238602-Chemotactic Factors,
pubmed-meshheading:11238602-Chemotaxis, Leukocyte,
pubmed-meshheading:11238602-Humans,
pubmed-meshheading:11238602-Hydroxyeicosatetraenoic Acids,
pubmed-meshheading:11238602-Injections, Intradermal,
pubmed-meshheading:11238602-Intracellular Fluid,
pubmed-meshheading:11238602-Lipoxins,
pubmed-meshheading:11238602-Mice,
pubmed-meshheading:11238602-Mice, Inbred BALB C,
pubmed-meshheading:11238602-Molecular Sequence Data,
pubmed-meshheading:11238602-Neutrophil Infiltration,
pubmed-meshheading:11238602-Neutrophils,
pubmed-meshheading:11238602-Opioid Peptides,
pubmed-meshheading:11238602-RNA, Messenger,
pubmed-meshheading:11238602-Receptors, Opioid
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pubmed:year |
2001
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pubmed:articleTitle |
Cutting edge: nociceptin stimulates neutrophil chemotaxis and recruitment: inhibition by aspirin-triggered-15-epi-lipoxin A4.
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pubmed:affiliation |
Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. cnserhan@zeus.bwh.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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