Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-3-12
pubmed:abstractText
Two approaches were used to correlate the Aedes aegypti genetic linkage map to the physical map. STS markers were developed for previously mapped RFLP-based genetic markers so that large genomic clones from cosmid libraries could be found and placed to the metaphase chromosome physical maps using standard FISH methods. Eight cosmids were identified that contained eight RFLP marker sequences, and these cosmids were located on the metaphase chromosomes. Twenty-one cDNAs were mapped directly to metaphase chromosomes using a FISH amplification procedure. The chromosome numbering schemes of the genetic linkage and physical maps corresponded directly and the orientations of the genetic linkage maps for chromosomes 2 and 3 were inverted relative to the physical maps. While the chromosome 2 linkage map represented essentially 100% of chromosome 2, approximately 65% of the chromosome 1 linkage map mapped to only 36% of the short p-arm and 83% of the chromosome 3 physical map contained the complete genetic linkage map. Since the genetic linkage map is a RFLP cDNA-based map, these data also provide a minimal estimate for the size of the euchromatic regions. The implications of these findings on positional cloning in A. aegypti are discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-15275410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-1763031, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-1802804, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-2052024, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-2470653, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7498432, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7505701, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7633400, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7789771, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7846038, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7931905, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-7987523, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8101854, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8342025, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8589842, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8630489, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8725240, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-8851807, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-9087552, http://linkedlifedata.com/resource/pubmed/commentcorrection/11238414-9099584
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1299-305
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Integration of the Aedes aegypti mosquito genetic linkage and physical maps.
pubmed:affiliation
Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't