Source:http://linkedlifedata.com/resource/pubmed/id/11237670
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2001-3-12
|
| pubmed:abstractText |
Cell-mediated gene therapy for visceral lesions of lysosomal storage diseases is promising; however, the treatment of central nervous system (CNS) lesions remains a challenge. In this study, we generated rat amniotic epithelial cells (AEC) that overexpress and secrete human beta-glucuronidase (GUSB) following transduction with an adenoviral vector encoding human GUSB. The AEC were used as donor cells for cell-mediated gene therapy of CNS lesions in mice with mucopolysaccharidosis type VII (MPSVII), a lysosomal storage disorder caused by an inherited deficiency of GUSB activity. After confirmation that the secreted GUSB was taken up mainly via mannose 6-phosphate receptors in primary cultured neurons, the AEC were transplanted into the brains of adult MPSVII mice. Histochemical analysis showed extensive GUSB activity throughout the ipsilateral hemisphere of the recipient brains, and pathological improvement of the lysosomal storage was observed even in regions far from the site of injection. These results suggest that intracerebral transplantation of genetically engineered AEC has therapeutic potential for the treatment of CNS lesions in lysosomal storage disorders.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1525-0016
|
| pubmed:author |
pubmed-author:AraiHH,
pubmed-author:AzumaNN,
pubmed-author:KamataYY,
pubmed-author:KosugaMM,
pubmed-author:LiX KXK,
pubmed-author:OginoII,
pubmed-author:OkawaHH,
pubmed-author:OkudaOO,
pubmed-author:OkuyamaTT,
pubmed-author:SakuragawaNN,
pubmed-author:SasakiKK,
pubmed-author:SuzukiSS,
pubmed-author:TanabeAA,
pubmed-author:YamadaMM
|
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
139-48
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11237670-Adenoviridae,
pubmed-meshheading:11237670-Amniotic Fluid,
pubmed-meshheading:11237670-Animals,
pubmed-meshheading:11237670-Brain,
pubmed-meshheading:11237670-Cells, Cultured,
pubmed-meshheading:11237670-Disease Models, Animal,
pubmed-meshheading:11237670-Epithelial Cells,
pubmed-meshheading:11237670-Gene Therapy,
pubmed-meshheading:11237670-Gene Transfer Techniques,
pubmed-meshheading:11237670-Genetic Vectors,
pubmed-meshheading:11237670-Glucuronidase,
pubmed-meshheading:11237670-Humans,
pubmed-meshheading:11237670-Lysosomes,
pubmed-meshheading:11237670-Mice,
pubmed-meshheading:11237670-Microscopy, Fluorescence,
pubmed-meshheading:11237670-Mucopolysaccharidosis VII,
pubmed-meshheading:11237670-Neurons,
pubmed-meshheading:11237670-Rats,
pubmed-meshheading:11237670-Rats, Wistar,
pubmed-meshheading:11237670-Receptor, IGF Type 2,
pubmed-meshheading:11237670-Time Factors,
pubmed-meshheading:11237670-Transduction, Genetic,
pubmed-meshheading:11237670-Transplantation
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Engraftment of genetically engineered amniotic epithelial cells corrects lysosomal storage in multiple areas of the brain in mucopolysaccharidosis type VII mice.
|
| pubmed:affiliation |
Department of Genetics, National Children's Medical Research Center, Tokyo, 154-8509, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|