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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1975-6-29
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pubmed:abstractText |
1. A new preparation of gastric mucosa isolated from new-born piglets is described. The piglet gastric mucosa was easily separated from the serosal muscle layers by a "blistering" technique which appeared to cause minimal trauma to the tissue and which allowed extended study in vitro in a suitable chamber. Normal resting p.d. was approximately minus 30 mV (mucosal side negative with respect to serosal side), resistance about 100 omega. cm-2 and H+ secretion was absent or occurred at very low rates (0-1mu-equiv/cm-2. hr). 2. Maximally stimulating doses of histamine (1-6 times 10-5 M) caused H+ secretion to increase (up to 15 muequiv/cm-2. hr), p.d. to increase and resistance to decrease. A close correlation was observed between the increase in H+ secretion and decrease in transmucosal resistance. The threshold dose of histamine appeared to be 10-8 M; concentrations 10-4 M and higher reduced H+ secretion somewhat. 3. Pentagastrin ( 10-9-10-7 M) and acetylcholine (10-7-10-5 M) did not significantly stimulate the piglet gastric mucosa. Pentagastrin concentrations above 4 times 10-6 M reversibly inhibited H+ secretion of histamine-stimulated mucosa. High concentrations of acetylcholine (above 4 times 10-4 M) did not affect histamine-stimulated H+ secretion, but a significant reduction in p.d. was observed. 4. This investigation demonstrates the utility of the piglet gastric mucosa for in vitro studies of the mechanism H+ secretion and the action of secretagogues. From a consideration of such factors as the thinness of tissue and ease of preparation it is suggested that neonatal animals may represent a good source of in vitro mammalian gastric tissue.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-1123757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-13030804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-13080432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-13327046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-13628626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-14001948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-14275518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-14286864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-14376639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-14491117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-15435709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-4320917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-4976023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5066487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5346308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5497554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5661089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5775893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5853154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-5959025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-6040431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1123738-6067479
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1123738-Acetylcholine,
pubmed-meshheading:1123738-Animals,
pubmed-meshheading:1123738-Animals, Newborn,
pubmed-meshheading:1123738-Depression, Chemical,
pubmed-meshheading:1123738-Dose-Response Relationship, Drug,
pubmed-meshheading:1123738-Electrophysiology,
pubmed-meshheading:1123738-Gastric Juice,
pubmed-meshheading:1123738-Gastric Mucosa,
pubmed-meshheading:1123738-Histamine,
pubmed-meshheading:1123738-Pentagastrin,
pubmed-meshheading:1123738-Stimulation, Chemical,
pubmed-meshheading:1123738-Swine
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pubmed:year |
1975
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pubmed:articleTitle |
Histamine-stimulated hydrogen ion secretion by in vitro piglet gastric mucosa.
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pubmed:publicationType |
Journal Article,
In Vitro
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