Source:http://linkedlifedata.com/resource/pubmed/id/11230330
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Part 2
|
| pubmed:dateCreated |
2001-3-20
|
| pubmed:abstractText |
There is evidence in humans that hypertension and aging similarly impair endothelial function, although the mechanism remains unclear. Superoxide anion (O(2)(-)) is a major determinant of nitric oxide (NO) bioavailability and thus endothelial function. We sought to determine the relationship between endothelial function, O(2)(-), and age in normotensive Wistar-Kyoto (WKY) and stroke-prone spontaneously hypertensive rats (SHRSP). Aortic rings were removed from female WKY and SHRSP at 3 to 4 months (young) and 9 to 12 months (old). O(2)(-) generation by aortic rings was measured before and after removal of the endothelium or incubation with N(G) nitro-L-arginine methyl ester, diphenyleneiodonium, or apocynin. Levels of p22phox were studied with immunohistochemistry and used as a marker of NAD(P)H oxidase expression. NO bioavailability was significantly lower in old WKY compared with young WKY (P=0.0009) and in old SHRSP compared with young SHRSP (P=0.005). O(2)(-) generation was significantly greater in old WKY compared with young WKY (P=0.0001). Removal of the endothelium and N(G) nitro-L-arginine methyl ester treatment resulted in a significant reduction in O(2)(-) generation in old SHRSP (P=0.009 and 0.001, respectively). Diphenyleneiodonium significantly reduced O(2)(-) generation in 12-month WKY (P=0.008) and 12-month SHRSP (P=0.009). Apocynin attenuated O(2)(-) generation by older WKY (P=0.038) and SHRSP (P=0.028). p22phox was increased in older animals compared with young. We conclude that NO bioavailability decreases with age in female WKY and SHRSP. O(2)(-) generation increases with age in WKY and is higher in SHRSP and may contribute to the reduced NO by scavenging. NAD(P)H oxidase may contribute to the age-related increase in O(2)(-).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetophenones,
http://linkedlifedata.com/resource/pubmed/chemical/CYBA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Onium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/acetovanillone,
http://linkedlifedata.com/resource/pubmed/chemical/diphenyleneiodonium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1524-4563
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
529-34
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:11230330-Acetophenones,
pubmed-meshheading:11230330-Aging,
pubmed-meshheading:11230330-Animals,
pubmed-meshheading:11230330-Aorta,
pubmed-meshheading:11230330-Blood Pressure,
pubmed-meshheading:11230330-Carotid Arteries,
pubmed-meshheading:11230330-Endothelium, Vascular,
pubmed-meshheading:11230330-Enzyme Inhibitors,
pubmed-meshheading:11230330-Female,
pubmed-meshheading:11230330-Hypertension,
pubmed-meshheading:11230330-Immunohistochemistry,
pubmed-meshheading:11230330-Luminescent Measurements,
pubmed-meshheading:11230330-Membrane Transport Proteins,
pubmed-meshheading:11230330-NADPH Dehydrogenase,
pubmed-meshheading:11230330-NADPH Oxidase,
pubmed-meshheading:11230330-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:11230330-Nitric Oxide,
pubmed-meshheading:11230330-Onium Compounds,
pubmed-meshheading:11230330-Phosphoproteins,
pubmed-meshheading:11230330-Rats,
pubmed-meshheading:11230330-Rats, Inbred SHR,
pubmed-meshheading:11230330-Rats, Inbred WKY,
pubmed-meshheading:11230330-Superoxides
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Superoxide excess in hypertension and aging: a common cause of endothelial dysfunction.
|
| pubmed:affiliation |
Department of Medicine and Therapeutics, Western Infirmary, Glasgow, UK. cah1p@clinmed.gla.ac.uk
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|