Source:http://linkedlifedata.com/resource/pubmed/id/11230115
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2001-3-20
|
| pubmed:abstractText |
In the absence of hypertrophic proliferation of microtubules, microtubule disruption by colchicine does not modulate contraction of adult cardiac myocytes. However, Gomez et al (Circ Res. 2000;86:30-36) recently reported that disruption of microtubules by colchicine in ruptured patch-clamped myocytes increased I(Ca,L) density and [Ca(2+)](i) transient amplitude and depressed the response of these parameters to the beta-adrenoceptor agonist isoproterenol. These effects were ascribed to stimulation of adenylyl cyclase by increased intracellular free tubulin. In the present study, we show that in intact rat ventricular myocytes, 2 to 4 hours of exposure to 10 micromol/L colchicine had no effect on shortening or [Ca(2+)](i) transient amplitude or on the amplitude of I(Ca,L) in perforated patch-clamped cells, under basal conditions and after stimulation with 1 micromol/L isoproterenol. However, in ruptured patch-clamped myocytes, basal I(Ca,L) was 2-fold higher after treatment with colchicine compared with vehicle and, in contrast to vehicle-treated cells, I(Ca,L) did not increase in response to isoproterenol. Cell width decreased during ruptured patch-clamp experiments in colchicine-treated but not vehicle-treated myocytes. We conclude that in cells with intact sarcolemma, colchicine does not modulate Ca(2+) signaling or the response to beta stimulation. However, the combination of microtubule disruption by colchicine and the ruptured patch configuration activates I(Ca,L) and attenuates the response to beta stimulation. We propose that these effects may be due to loss of free tubulin by intracellular dialysis or to increased sensitivity to mechanical stimulation as a result of microtubule disruption. These findings have important implications for cardiomyopathies associated with decreased free tubulin or a diminished microtubular network. The full text of this article is available at http://www.circresaha.org.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amphotericin B,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Paclitaxel
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1524-4571
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
2
|
| pubmed:volume |
88
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
E32-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:11230115-Amphotericin B,
pubmed-meshheading:11230115-Animals,
pubmed-meshheading:11230115-Calcium,
pubmed-meshheading:11230115-Calcium Channels,
pubmed-meshheading:11230115-Cell Division,
pubmed-meshheading:11230115-Cell Size,
pubmed-meshheading:11230115-Colchicine,
pubmed-meshheading:11230115-Fluorescent Antibody Technique,
pubmed-meshheading:11230115-Heart Ventricles,
pubmed-meshheading:11230115-Ion Channel Gating,
pubmed-meshheading:11230115-Isoproterenol,
pubmed-meshheading:11230115-Male,
pubmed-meshheading:11230115-Microscopy, Confocal,
pubmed-meshheading:11230115-Microtubules,
pubmed-meshheading:11230115-Myocardial Contraction,
pubmed-meshheading:11230115-Myocardium,
pubmed-meshheading:11230115-Paclitaxel,
pubmed-meshheading:11230115-Patch-Clamp Techniques,
pubmed-meshheading:11230115-Rats,
pubmed-meshheading:11230115-Rats, Wistar,
pubmed-meshheading:11230115-Signal Transduction
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Modulation of Ca(2+) signaling by microtubule disruption in rat ventricular myocytes and its dependence on the ruptured patch-clamp configuration.
|
| pubmed:affiliation |
School of Biomedical Sciences, University of Leeds, Leeds, UK.
|
| pubmed:publicationType |
Journal Article
|