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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-3-2
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pubmed:abstractText |
E-selectin, exclusively expressed on activated endothelial cells, is a potential target for site-directed delivery of agents. We and others have shown that sialyl LewisX-liposomes (sLe(x)-liposomes) are recognized by E-selectin. We now report an approach employing sLe(x)-liposomes to deliver antisense oligonucleotides (AS-ODNs) directed against the adhesion molecule ICAM-1 to activated vascular endothelial cells. ICAM-1 expression was analyzed at the protein level by immunofluorescence and a cell surface ELISA, and at the RNA level by RT-PCR. We have investigated two different AS-ODNs complementary to the 3' untranslated region and the AUG translation initiation codon of ICAM-1 mRNA. Both inhibited protein expression, but did not influence the mRNA level, pointing to a hybridization of AS-ODNs with the mRNA in the cytoplasm. Our results demonstrate the feasibility of a novel approach for the delivery of agents to activated endothelial cells by glycoliposomes targeted to E-selectin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-acetylneuraminyl-(2-3)-galactosyl-...,
http://linkedlifedata.com/resource/pubmed/chemical/Codon, Initiator,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1420-682X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11229813-Cells, Cultured,
pubmed-meshheading:11229813-Codon, Initiator,
pubmed-meshheading:11229813-Dose-Response Relationship, Drug,
pubmed-meshheading:11229813-Down-Regulation,
pubmed-meshheading:11229813-Drug Delivery Systems,
pubmed-meshheading:11229813-E-Selectin,
pubmed-meshheading:11229813-Endothelium, Vascular,
pubmed-meshheading:11229813-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11229813-Fluorescent Antibody Technique,
pubmed-meshheading:11229813-Humans,
pubmed-meshheading:11229813-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11229813-Liposomes,
pubmed-meshheading:11229813-Oligonucleotides, Antisense,
pubmed-meshheading:11229813-Oligosaccharides,
pubmed-meshheading:11229813-Organ Specificity,
pubmed-meshheading:11229813-Protein Binding,
pubmed-meshheading:11229813-RNA, Messenger,
pubmed-meshheading:11229813-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:year |
2001
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pubmed:articleTitle |
Sialyl Lewis(x)-liposomes as vehicles for site-directed, E-selectin-mediated drug transfer into activated endothelial cells.
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pubmed:affiliation |
Max Delbrück Center for Molecular Medicine, Berlin, Germany. renate.stahn@nemod.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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