Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-3-6
pubmed:abstractText
Nitric oxide (NO) plays a critical role in vascular endothelial growth factor (VEGF)-induced angiogenesis and vascular hyperpermeability. However, the relative contribution of different NO synthase (NOS) isoforms to these processes is not known. Here, we evaluated the relative contributions of endothelial and inducible NOS (eNOS and iNOS, respectively) to angiogenesis and permeability of VEGF-induced angiogenic vessels. The contribution of eNOS was assessed by using an eNOS-deficient mouse, and iNOS contribution was assessed by using a selective inhibitor [l-N(6)-(1-iminoethyl) lysine, l-NIL] and an iNOS-deficient mouse. Angiogenesis was induced by VEGF in type I collagen gels placed in the mouse cranial window. Angiogenesis, vessel diameter, blood flow rate, and vascular permeability were proportional to NO levels measured with microelectrodes: Wild-type (WT) > or = WT with l-NIL or iNOS(-/-) > eNOS(-/-) > or = eNOS(-/-) with l-NIL. The role of NOS in VEGF-induced acute vascular permeability increase in quiescent vessels also was determined by using eNOS- and iNOS-deficient mice. VEGF superfusion significantly increased permeability in both WT and iNOS(-/-) mice but not in eNOS(-/-) mice. These findings suggest that eNOS plays a predominant role in VEGF-induced angiogenesis and vascular permeability. Thus, selective modulation of eNOS activity is a promising strategy for altering angiogenesis and vascular permeability in vivo.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10329591, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10329964, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10376602, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10455194, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10463618, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10469350, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10502827, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10516200, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10598133, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-10742145, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-11001068, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-1279431, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-1539722, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-1547488, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-3495737, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-6253831, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7500013, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7522969, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7525961, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7542286, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7545787, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-7990724, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8605350, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8666908, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8686763, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8691063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8812749, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-8997338, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9033284, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9096405, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9169492, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9250168, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9399960, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9443437, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9486966, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9529250, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9530221, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9544424, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9580259, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9616228, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9753319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9794766, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9804796, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226286-9837777
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III, http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Nos3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2604-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...