Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-3-15
pubmed:abstractText
Ca(2+)-ATPases are likely to play critical roles in the biochemistry of Toxoplasma gondii, since these protozoa are obligate intracellular parasites and the Ca(2+) concentration in their intracellular location is three orders of magnitude lower than in the extracellular medium. Here, we report the cloning and sequencing of a gene encoding a plasma membrane-type Ca(2+)-ATPase (PMCA) of T.gondii (TgA1). The predicted protein (TgA1) exhibits 32-36% identity to vacuolar Ca(2+)-ATPases of Trypanosoma cruzi, Saccharomyces cerevisiae, Entamoeba histolytica and Dictyostelium discoideum. Sequencing of both cDNA and genomic DNA from T.gondii indicated that TgA1 contains two introns near the C-terminus. A hydropathy profile of the protein suggests 10 transmembrane domains. TgA1 suppresses the Ca(2+) hypersensitivity of a mutant of S.cerevisiae that has a defect in vacuolar Ca(2+) accumulation. Indirect immunofluorescence and immunoelectron microscopy analysis indicate that TgA1 localizes to the plasma membrane and co-localizes with the vacuolar H(+)-pyrophosphatase to intracellular vacuoles identified morphologically and by X-ray microanalysis as the acidocalcisomes. This vacuolar-type Ca(2+)-ATPase could play an important role in Ca(2+) homeostasis in T.gondii.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10027960, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10359662, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10455025, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10494806, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10498186, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10511686, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10523660, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10559249, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10613696, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10743619, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10802325, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10872220, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-10903134, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-131039, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-13844756, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-1431065, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-1561104, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-1826932, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-2137451, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-2645293, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-2844598, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-2887455, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-3028470, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-3412377, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-5342521, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-6451451, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-6791577, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7046728, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7108955, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7499325, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7507493, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7575396, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7575411, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7757057, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7836277, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7982261, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-7998937, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-8573106, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-8628289, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-8670117, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-8822901, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-8858595, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9083086, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9089733, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9208224, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9346954, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9480901, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9528801, http://linkedlifedata.com/resource/pubmed/commentcorrection/11226155-9705361
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-64
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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