Linked Life Data

11225849

Source:http://linkedlifedata.com/resource/pubmed/id/11225849

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-2-27
pubmed:abstractText
Upregulation of the p53 tumor suppressor protein by infection with a recombinant p53 adenovirus resulted in extensive apoptosis in ECV-304 cells and the eventual death of almost all the cells. To establish a system to elucidate the molecular mechanisms involved in p53-mediated apoptosis of these cells, we established a variant of ECV-304 that is resistant to p53-induced apoptosis by repeated infections with a recombinant p53 adenovirus. We have designated this variant as the DECV cell line (Differentiated ECV-304). DECV cells expressed similar amounts of nuclear-localized p53 as ECV-304 cells when infected with recombinant p53 adenovirus, but in contrast to ECV-304 cells, greater than 95% of DECV cells survived and remained viable after 24 hours of infection. In further contrast to ECV-304 cells, DECV cells grew less efficiently in soft agar and exhibited contact inhibition in growth assays. Moreover, DECV cells formed unusual lattice or cyst-like structures in culture and formed lumenal structures indicative of epithelial differentiation in three-dimensional collagen matrices, while parental ECV-304 cells showed minimal evidence of these cellular behaviors. A comparative molecular analysis of gene expression in DECV and ECV-304 cells was conducted by cDNA microarray technology. Protocadherin-1 was found to be expressed in DECV cells but not in ECV-304 cells, while the Id-3 gene was observed expressed in ECV-304 cells but not in DECV cells. Moreover, upregulated expression of p53 in ECV-304 cells induced the EPHB2 (Ephrin) receptor tyrosine kinase and the ephrin-B1 ligand mRNAs compared to DECV cells treated in the same manner. These data demonstrate that a new variant of the ECV-304 cell line, which is resistant to p53-mediated apoptosis, exhibits differential gene expression as well as distinct cell behaviors as compared to the parental ECV-304 cell line. DECV cells should prove to be a useful tool in future studies to elucidate mechanisms of p53-mediated apoptosis and differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1360-8185
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11225849-Adenoviridae, pubmed-meshheading:11225849-Apoptosis, pubmed-meshheading:11225849-Cadherins, pubmed-meshheading:11225849-Cell Differentiation, pubmed-meshheading:11225849-Cell Separation, pubmed-meshheading:11225849-Cell Size, pubmed-meshheading:11225849-Ephrin-B1, pubmed-meshheading:11225849-Flow Cytometry, pubmed-meshheading:11225849-Gene Expression Profiling, pubmed-meshheading:11225849-Genes, Reporter, pubmed-meshheading:11225849-Humans, pubmed-meshheading:11225849-Inhibitor of Differentiation Proteins, pubmed-meshheading:11225849-Membrane Proteins, pubmed-meshheading:11225849-Neoplasm Proteins, pubmed-meshheading:11225849-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11225849-Recombinant Fusion Proteins, pubmed-meshheading:11225849-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11225849-Transcription Factors, pubmed-meshheading:11225849-Tumor Cells, Cultured, pubmed-meshheading:11225849-Tumor Suppressor Protein p53
pubmed:year
2000
pubmed:articleTitle
Biological and molecular characterization of an ECV-304-derived cell line resistant to p53-mediated apoptosis.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, College of Medicine, Texas A&M University System Health Science Center, College Station 77843-1114, USA. s-maxwell@tamu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.