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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
We have previously shown that Y box-binding protein-1 (YB-1) binds preferentially to cisplatin-modified Y box sequences. Based on structural and biochemical data, we predicted that this protein binds single-stranded nucleic acids. In the present study we confirmed the prediction and also discovered some unexpected functional features of YB-1. We found that the cold shock domain of the protein is necessary but not sufficient for double-stranded DNA binding while the C-tail domain interacts with both single-stranded DNA and RNA independently of the cold shock domain. In an in vitro translation system the C-tail domain of the protein inhibited translation but the cold shock domain did not. Both in vitro pull-down and in vivo co-immunoprecipitation assays revealed that YB-1 can form a homodimer. Deletion analysis mapped the C-tail domain of the protein as the region of homodimerization. We also characterized an intrinsic 3'-->5' DNA exonuclease activity of the protein. The region between residues 51 and 205 of its 324-amino acid extent is required for full exonuclease activity. Our findings suggest that YB-1 functions in regulating DNA/RNA transactions and that these actions involve different domains.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10080895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10216949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10558877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10559330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10597187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10626815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10713044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10716181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10812007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-10817758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-1622927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-1622930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-1901052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-1923758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-1967130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-2977358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-3174636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-7499328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-7524668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-7530842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-7637031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-7702598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8004679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8031301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8321222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8625735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8674115,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8709216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8797596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8798444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-8967899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9095180,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9207042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9371456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9409758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9452484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9472015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9484881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9690508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9748149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9753699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9757828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9830047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222770-9927044
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Exonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1362-4962
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pubmed:author |
pubmed-author:DoraG LGL,
pubmed-author:ImamuraTT,
pubmed-author:IseTT,
pubmed-author:IshiguchiHH,
pubmed-author:IzumiHH,
pubmed-author:KohnoKK,
pubmed-author:KuwanoMM,
pubmed-author:MurakamiTT,
pubmed-author:NagataniGG,
pubmed-author:NomotoMM,
pubmed-author:OkamotoTT,
pubmed-author:TorigoeTT,
pubmed-author:UchiumiTT,
pubmed-author:UramotoHH,
pubmed-author:YoshidaYY
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1200-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
