| pubmed-article:11222688 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11222688 | lifeskim:mentions | umls-concept:C0035866 | lld:lifeskim |
| pubmed-article:11222688 | lifeskim:mentions | umls-concept:C0035366 | lld:lifeskim |
| pubmed-article:11222688 | lifeskim:mentions | umls-concept:C0598091 | lld:lifeskim |
| pubmed-article:11222688 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:11222688 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11222688 | pubmed:dateCreated | 2001-3-6 | lld:pubmed |
| pubmed-article:11222688 | pubmed:abstractText | Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the RRV glycoprotein-pseudotyped retroviruses for prolonged periods of time have been constructed. The pseudotyped viruses have a broadened host range, can be concentrated to high titer, and mediate stable transduction of genes into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to demonstrate that RRV glycoprotein-mediated viral entry occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses have significant advantages as reagents for the study of the biochemistry and prevention of alphavirus entry and as preferred vectors for stable gene transfer and gene therapy protocols. | lld:pubmed |
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| pubmed-article:11222688 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11222688 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11222688 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11222688 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11222688 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11222688 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:11222688 | pubmed:author | pubmed-author:SandersD ADA | lld:pubmed |
| pubmed-article:11222688 | pubmed:author | pubmed-author:KuhnR JRJ | lld:pubmed |
| pubmed-article:11222688 | pubmed:author | pubmed-author:NorthC LCL | lld:pubmed |
| pubmed-article:11222688 | pubmed:author | pubmed-author:SharkeyC MCM | lld:pubmed |
| pubmed-article:11222688 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11222688 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:11222688 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11222688 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11222688 | pubmed:pagination | 2653-9 | lld:pubmed |
| pubmed-article:11222688 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11222688 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11222688 | pubmed:articleTitle | Ross River virus glycoprotein-pseudotyped retroviruses and stable cell lines for their production. | lld:pubmed |
| pubmed-article:11222688 | pubmed:affiliation | Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA. | lld:pubmed |
| pubmed-article:11222688 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11222688 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11222688 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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