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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the RRV glycoprotein-pseudotyped retroviruses for prolonged periods of time have been constructed. The pseudotyped viruses have a broadened host range, can be concentrated to high titer, and mediate stable transduction of genes into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to demonstrate that RRV glycoprotein-mediated viral entry occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses have significant advantages as reagents for the study of the biochemistry and prevention of alphavirus entry and as preferred vectors for stable gene transfer and gene therapy protocols.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-10473628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-1370493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-1433520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-1673812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-1695253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-2156225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-2408229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-2448952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-2500008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-2831375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-3102226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-3829124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-6247355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-6492263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-6991511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7479858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7690960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7774013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7867069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7937806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7954815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-7968923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-8350409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-8415660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-8602510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-8642678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-8876147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9188652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9242523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9426049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9461435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9525641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9707418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11222688-9721245
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2653-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11222688-3T3 Cells,
pubmed-meshheading:11222688-Animals,
pubmed-meshheading:11222688-Capsid,
pubmed-meshheading:11222688-Capsid Proteins,
pubmed-meshheading:11222688-Cell Fusion,
pubmed-meshheading:11222688-Cell Line,
pubmed-meshheading:11222688-Genetic Vectors,
pubmed-meshheading:11222688-Hydrogen-Ion Concentration,
pubmed-meshheading:11222688-Membrane Fusion,
pubmed-meshheading:11222688-Membrane Glycoproteins,
pubmed-meshheading:11222688-Mice,
pubmed-meshheading:11222688-Moloney murine leukemia virus,
pubmed-meshheading:11222688-Recombinant Fusion Proteins,
pubmed-meshheading:11222688-Ross River virus,
pubmed-meshheading:11222688-Transduction, Genetic,
pubmed-meshheading:11222688-Viral Envelope Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Ross River virus glycoprotein-pseudotyped retroviruses and stable cell lines for their production.
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pubmed:affiliation |
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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