|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-3-6
|
|
pubmed:abstractText |
The CD94/NKG2A heterodimer is an inhibitory receptor expressed on a subset of mouse NK cells. CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1(b) and inhibits NK cytotoxicity. Qa-1(b) presents a peptide derived from the leader sequence of classical MHC class I molecules. Here, we examined the role of CD94/NKG2A in T cell-mediated cytotoxicity. Soluble tetrameric Qa-1(b) bound to almost all CD8(+), but not CD4(+), T cells. This binding seems to be mediated by CD8, because COS cells transfected with CD8 also bound Qa-1(b) tetramer. Therefore, the expression of CD94/NKG2 in T cells was further examined by single-cell RT-PCR. Most murine CD8(+) T cells constitutively expressed CD94 and NKG2A transcripts, whereas they were not detected in CD4(+) T cells. Co-expression of Qa-1(b) and D(k) on target cells significantly inhibited cytotoxicity of D(k)-specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction, indicating that Qa-1(b) on antigen-presenting cells interacts with CD94/NKG2A on CD8 T cells and regulates classical MHC class I-restricted cytotoxic T cells. These results suggest a significant role of CD94/NKG2A as an inhibitory receptor on CD8(+) T cells.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Klrc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Klrd1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Klrd1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Q surface antigens,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Natural Killer Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/histocompatibility antigen H-2D(b)
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0953-8178
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
13
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
321-7
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:11222501-Animals,
pubmed-meshheading:11222501-Antigens, CD,
pubmed-meshheading:11222501-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11222501-CD8-Positive T-Lymphocytes,
pubmed-meshheading:11222501-COS Cells,
pubmed-meshheading:11222501-Cercopithecus aethiops,
pubmed-meshheading:11222501-Cytotoxicity, Immunologic,
pubmed-meshheading:11222501-Dimerization,
pubmed-meshheading:11222501-Genes, MHC Class I,
pubmed-meshheading:11222501-H-2 Antigens,
pubmed-meshheading:11222501-Histocompatibility Antigens Class I,
pubmed-meshheading:11222501-Lectins, C-Type,
pubmed-meshheading:11222501-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:11222501-Membrane Glycoproteins,
pubmed-meshheading:11222501-Mice,
pubmed-meshheading:11222501-Mice, Inbred C3H,
pubmed-meshheading:11222501-Mice, Inbred C57BL,
pubmed-meshheading:11222501-NK Cell Lectin-Like Receptor Subfamily C,
pubmed-meshheading:11222501-NK Cell Lectin-Like Receptor Subfamily D,
pubmed-meshheading:11222501-RNA, Messenger,
pubmed-meshheading:11222501-Rats,
pubmed-meshheading:11222501-Receptors, Immunologic,
pubmed-meshheading:11222501-Receptors, Natural Killer Cell,
pubmed-meshheading:11222501-Recombinant Fusion Proteins,
pubmed-meshheading:11222501-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11222501-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11222501-Transfection
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
The non-classical MHC class I molecule Qa-1(b) inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes.
|
|
pubmed:affiliation |
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3 Canada.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
