Source:http://linkedlifedata.com/resource/pubmed/id/11222393
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
The dehydration of sickle red blood cells (RBCs) through the Ca-activated K channel depends on the parallel movement of Cl ions. To study whether Cl-conductance block might prevent dehydration of sickle RBCs, a novel Cl-conductance inhibitor (NS3623) was characterized in vitro using RBCs from healthy donors and sickle cell patients and in vivo using normal mice and a transgenic mouse model of sickle cell disease (SAD mice). In vitro, NS3623 reversibly blocked human RBC Cl-conductance (g(Cl)) with an IC(50) value of 210 nmol/L and a maximal block of 95%. In vivo, NS3623 inhibited RBC g(Cl) after oral administration to normal mice (ED(50) = 25 mg/kg). Although g(Cl), at a single dose of 100 mg/kg, was still 70% inhibited 5 hours after dosing, the inhibition disappeared after 24 hours. Repeated administration of 100 mg/kg twice a day for 10 days caused no adverse effects; therefore, this regimen was chosen as the highest dosing for the SAD mice. SAD mice were treated for 3 weeks with 2 daily administrations of 10, 35, and 100 mg/kg NS3623, respectively. The hematocrit increased, and the mean corpuscular hemoglobin concentration decreased in all groups with a concomitant increase in the intracellular cation content. A loss of the densest red cell population was observed in conjunction with a shift from a high proportion of sickled to well-hydrated discoid erythrocytes, with some echinocytes present at the highest dosage. These data indicate feasibility for the potential use of Cl-conductance blockers to treat human sickle cell disease.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobin, Sickle,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxyhemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylurea Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/oxyhemoglobin S
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1451-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11222393-Anemia, Sickle Cell,
pubmed-meshheading:11222393-Animals,
pubmed-meshheading:11222393-Chloride Channels,
pubmed-meshheading:11222393-Dehydration,
pubmed-meshheading:11222393-Disease Models, Animal,
pubmed-meshheading:11222393-Dose-Response Relationship, Drug,
pubmed-meshheading:11222393-Erythrocytes,
pubmed-meshheading:11222393-Hematocrit,
pubmed-meshheading:11222393-Hemoglobin, Sickle,
pubmed-meshheading:11222393-Hemoglobins,
pubmed-meshheading:11222393-Humans,
pubmed-meshheading:11222393-Mice,
pubmed-meshheading:11222393-Mice, Transgenic,
pubmed-meshheading:11222393-Oxyhemoglobins,
pubmed-meshheading:11222393-Phenylurea Compounds,
pubmed-meshheading:11222393-Tetrazoles,
pubmed-meshheading:11222393-Time Factors,
pubmed-meshheading:11222393-Water
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pubmed:year |
2001
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pubmed:articleTitle |
Treatment with NS3623, a novel Cl-conductance blocker, ameliorates erythrocyte dehydration in transgenic SAD mice: a possible new therapeutic approach for sickle cell disease.
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pubmed:affiliation |
August Krogh Institute, University of Copenhagen, and NeuroSearch A/S, Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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