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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-2-23
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pubmed:abstractText |
KVLQT1 (KCNQ1) is a voltage-gated K+ channel essential for repolarization of the heart action potential that is defective in cardiac arrhythmia. The channel is inhibited by the chromanol 293B, a compound that blocks cAMP-dependent electrolyte secretion in rat and human colon, therefore suggesting expression of a similar type of K+ channel in the colonic epithelium. We now report cloning and expression of KVLQT1 from rat colon. Overlapping clones identified by cDNA-library screening were combined to a full length cDNA that shares high sequence homology to KVLQT1 cloned from other species. RT-PCR analysis of rat colonic musoca demonstrated expression of KVLQT1 in crypt cells and surface epithelium. Expression of rKVLQT1 in Xenopus oocytes induced a typical delayed activated K+ current, that was further activated by increase of intracellular cAMP but not Ca2+ and that was blocked by the chromanol 293B. The same compound blocked a basolateral cAMP-activated K+ conductance in the colonic mucosal epithelium and inhibited whole cell K+ currents in patch-clamp experiments on isolated colonic crypts. We conclude that KVLQT1 is forming an important component of the basolateral cAMP-activated K+ conductance in the colonic epithelium and plays a crucial role in diseases like secretory diarrhea and cystic fibrosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Barium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/KCNQ Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/KCNQ1 Potassium Channel,
http://linkedlifedata.com/resource/pubmed/chemical/KCNQ1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kcnq1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Voltage-Gated,
http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-2631
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
155-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11220365-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:11220365-Amino Acid Sequence,
pubmed-meshheading:11220365-Animals,
pubmed-meshheading:11220365-Barium,
pubmed-meshheading:11220365-Carbachol,
pubmed-meshheading:11220365-Cardiotonic Agents,
pubmed-meshheading:11220365-Cloning, Molecular,
pubmed-meshheading:11220365-Colon,
pubmed-meshheading:11220365-Cyclic AMP,
pubmed-meshheading:11220365-Dinoprostone,
pubmed-meshheading:11220365-Forskolin,
pubmed-meshheading:11220365-Humans,
pubmed-meshheading:11220365-Intestinal Mucosa,
pubmed-meshheading:11220365-KCNQ Potassium Channels,
pubmed-meshheading:11220365-KCNQ1 Potassium Channel,
pubmed-meshheading:11220365-Male,
pubmed-meshheading:11220365-Molecular Sequence Data,
pubmed-meshheading:11220365-Oocytes,
pubmed-meshheading:11220365-Patch-Clamp Techniques,
pubmed-meshheading:11220365-Phosphodiesterase Inhibitors,
pubmed-meshheading:11220365-Potassium Channels,
pubmed-meshheading:11220365-Potassium Channels, Voltage-Gated,
pubmed-meshheading:11220365-Rats,
pubmed-meshheading:11220365-Rats, Wistar,
pubmed-meshheading:11220365-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11220365-Sequence Alignment,
pubmed-meshheading:11220365-Tetraethylammonium,
pubmed-meshheading:11220365-Xenopus laevis
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pubmed:year |
2001
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pubmed:articleTitle |
Cloning and function of the rat colonic epithelial K+ channel KVLQT1.
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pubmed:affiliation |
Department of Physiology & Pharmacology, University of Queensland St Lucia, Brisbane, Australia.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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