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pubmed-article:11219738pubmed:dateCreated2001-2-21lld:pubmed
pubmed-article:11219738pubmed:abstractTextA microfabricated amperometric microcell was designed and used for the determination of proline iminopeptidase (PIP) enzyme activity in 2-10-microl samples. The measurements were made in the range of 10.3-841.5 mU/ml enzyme activities. The sensitivity of the determinations was between - 0.0195 and - 0.0203 microA ml/mU per min. The coefficient of variation of the determined values ranged between 2.8 (at 561.2 mU/ml) and 24.1% (at 10.3 mU/ml). The microcell was manufactured on an alumina substrate using screen-printed graphite working and Ag/AgCl reference electrodes. Elevated PIP activity in the vaginal fluid is a biochemical indicator of bacterial vaginosis. The method is appropriate to differentiate between normal (66+/-145 mU/ml) and elevated, diseased (704+/-145 mU/ml), values.lld:pubmed
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pubmed-article:11219738pubmed:authorpubmed-author:NeumanM RMRlld:pubmed
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pubmed-article:11219738pubmed:pagination265-72lld:pubmed
pubmed-article:11219738pubmed:dateRevised2009-7-14lld:pubmed
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pubmed-article:11219738pubmed:year2000lld:pubmed
pubmed-article:11219738pubmed:articleTitleScreen-printed amperometric microcell for proline iminopeptidase enzyme activity assay.lld:pubmed
pubmed-article:11219738pubmed:affiliationJoint Graduate Program in Biomedical Engineering, The University of Memphis and University of Tennessee Health Science Center, Herff College of Engineering, 38152-6582, USA.lld:pubmed
pubmed-article:11219738pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11219738pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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