Source:http://linkedlifedata.com/resource/pubmed/id/11219738
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
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pubmed:dateCreated |
2001-2-21
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pubmed:abstractText |
A microfabricated amperometric microcell was designed and used for the determination of proline iminopeptidase (PIP) enzyme activity in 2-10-microl samples. The measurements were made in the range of 10.3-841.5 mU/ml enzyme activities. The sensitivity of the determinations was between - 0.0195 and - 0.0203 microA ml/mU per min. The coefficient of variation of the determined values ranged between 2.8 (at 561.2 mU/ml) and 24.1% (at 10.3 mU/ml). The microcell was manufactured on an alumina substrate using screen-printed graphite working and Ag/AgCl reference electrodes. Elevated PIP activity in the vaginal fluid is a biochemical indicator of bacterial vaginosis. The method is appropriate to differentiate between normal (66+/-145 mU/ml) and elevated, diseased (704+/-145 mU/ml), values.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0956-5663
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-72
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:11219738-Aminopeptidases,
pubmed-meshheading:11219738-Biosensing Techniques,
pubmed-meshheading:11219738-Body Fluids,
pubmed-meshheading:11219738-Electrochemistry,
pubmed-meshheading:11219738-Female,
pubmed-meshheading:11219738-Humans,
pubmed-meshheading:11219738-Pregnancy,
pubmed-meshheading:11219738-Pregnancy Complications, Infectious,
pubmed-meshheading:11219738-Vagina,
pubmed-meshheading:11219738-Vaginosis, Bacterial
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pubmed:year |
2000
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pubmed:articleTitle |
Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay.
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pubmed:affiliation |
Joint Graduate Program in Biomedical Engineering, The University of Memphis and University of Tennessee Health Science Center, Herff College of Engineering, 38152-6582, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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