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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-2-9
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pubmed:abstractText |
Ex vivo ELISPOT analysis of peripheral blood lymphocytes obtained from stage IV melanoma patients demonstrated reactivity against peptides derived from MART-1 and gp100. However, the number of reactive T cells was < 1% that of total lymphocytes as detected by flow cytometry using tetrameric MHC/peptide complexes. Despite this low frequency, we were able to directly isolate these populations ex vivo by means of magnetic beads coated with MHC/peptide complexes and to subject these cells to T-cell receptor clonotype mapping. This analysis revealed that the MART-1/A*0201- and gp100/A*0201-reactive T-cell populations are composed of oligoclonal T cells that engage several T-cell receptor beta chain families. Longitudinal studies using this approach may result in a better correlation between T-cell reactivity and the course of neoplastic disease.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/SILV protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/gp100 Melanoma Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
493-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11212239-Aged,
pubmed-meshheading:11212239-Antigens, Neoplasm,
pubmed-meshheading:11212239-Clone Cells,
pubmed-meshheading:11212239-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11212239-Epitopes, T-Lymphocyte,
pubmed-meshheading:11212239-Flow Cytometry,
pubmed-meshheading:11212239-HLA-A Antigens,
pubmed-meshheading:11212239-Humans,
pubmed-meshheading:11212239-MART-1 Antigen,
pubmed-meshheading:11212239-Male,
pubmed-meshheading:11212239-Melanoma,
pubmed-meshheading:11212239-Membrane Glycoproteins,
pubmed-meshheading:11212239-Middle Aged,
pubmed-meshheading:11212239-Neoplasm Proteins,
pubmed-meshheading:11212239-Neoplasm Staging,
pubmed-meshheading:11212239-RNA, Neoplasm,
pubmed-meshheading:11212239-Receptors, Antigen, T-Cell,
pubmed-meshheading:11212239-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:11212239-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11212239-T-Lymphocytes,
pubmed-meshheading:11212239-Tumor Markers, Biological,
pubmed-meshheading:11212239-gp100 Melanoma Antigen
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pubmed:year |
2001
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pubmed:articleTitle |
Oligoclonal T-cell receptor usage of melanocyte differentiation antigen-reactive T cells in stage IV melanoma patients.
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pubmed:affiliation |
Department of Dermatology, Julius Maximilians-University, Würzburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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