Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-2-7
pubmed:databankReference
pubmed:abstractText
Bim is a proapoptotic protein of the Bcl-2 family that shares only the short BH3 domain with other members. It has three isoforms, apparently produced by alternative splicing. The demonstration that Bim is essential for certain apoptotic responses and to prevent overproduction of hematopoietic cells suggests that it may be a tumor suppressor. We have, therefore, investigated the organization of the mouse Bim gene, delineating its promoter and splicing, and positioned the gene on both mouse and human chromosomes. Bim has six exons, but the third is a facultative intron that is spliced out in the mRNAs for the smaller isoforms (BimL and BimS), but not that encoding the largest isoform (BimEL). The 0.8-kb region 5' to exon 1, which contains a TATA-less promoter and binding sites for several transcription factors, can drive expression of a reporter gene. Mouse Bim localizes to the distal third of Chromosome (Chr) 2, near the F-G boundary, and its human counterpart to Chr 2q12 or q13. Deletions of these bands have been reported in ten tumors (eight hematopoietic), reinforcing the possibility that Bim is a tumor suppressor. These findings should help to clarify the regulation of Bim expression and to assess whether mutations involving Bim contribute to neoplastic and other diseases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0938-8990
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11210187-Alternative Splicing, pubmed-meshheading:11210187-Animals, pubmed-meshheading:11210187-Apoptosis, pubmed-meshheading:11210187-Apoptosis Regulatory Proteins, pubmed-meshheading:11210187-Base Sequence, pubmed-meshheading:11210187-Blotting, Northern, pubmed-meshheading:11210187-Carrier Proteins, pubmed-meshheading:11210187-Chromosomes, Human, Pair 2, pubmed-meshheading:11210187-Exons, pubmed-meshheading:11210187-Humans, pubmed-meshheading:11210187-In Situ Hybridization, Fluorescence, pubmed-meshheading:11210187-Introns, pubmed-meshheading:11210187-Male, pubmed-meshheading:11210187-Membrane Proteins, pubmed-meshheading:11210187-Mice, pubmed-meshheading:11210187-Molecular Sequence Data, pubmed-meshheading:11210187-Physical Chromosome Mapping, pubmed-meshheading:11210187-Promoter Regions, Genetic, pubmed-meshheading:11210187-Protein Isoforms, pubmed-meshheading:11210187-Proto-Oncogene Proteins, pubmed-meshheading:11210187-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:11210187-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2001
pubmed:articleTitle
Gene structure alternative splicing, and chromosomal localization of pro-apoptotic Bcl-2 relative Bim.
pubmed:affiliation
The Walter and Eliza Hall Institute of Medical Research, The Royal Melbourne Hospital, Parkville, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't