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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1975-6-26
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pubmed:abstractText |
A specific assay for determination of carbamyl phosphate (carbamyl P) in blood has been developed using the enzymatic conversion of carbamyl P to citrulline by ornithine carbamyl transferase (OCT). This assay, for evaluation of bioavailability of carbamyl P, is necessary for study of the in vivo and in vitro effects of carbamyl P as an antisickling agent. The recovery of carbamyl P as citrulline was 88 to 92 per cent. Carbamyl P in whole dog blood in vitro decomposed with first-order kinetics with a t-1/2 of 42 minutes, the same as the t-1/2 reported for decomposition of carbamyl P to cyanate in buffers at physiologic temperature and pH. When carbamyl P (190 mg. per kilogram) was injected intraperitoneally into mice, it appeared in the blood, with a maximum concentration of 0.2 mM observed between 1 and 5 minutes after injection, followed by a rapid decrease within 10 minutes. The clearance of carbamyl P from dog blood after intravenous injection was first-order, with a t-1/2 of approximately 2.2 minutes. The rapid disappearance of carbamyl P in vivo may possibly by explained by the action of tissue acyl phosphatases which have been reported to hydrolyze carbamyl P.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-2143
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
694-702
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1120935-Animals,
pubmed-meshheading:1120935-Biological Availability,
pubmed-meshheading:1120935-Carbamates,
pubmed-meshheading:1120935-Citrulline,
pubmed-meshheading:1120935-Dogs,
pubmed-meshheading:1120935-Kinetics,
pubmed-meshheading:1120935-Mice,
pubmed-meshheading:1120935-Mice, Inbred BALB C,
pubmed-meshheading:1120935-Mice, Inbred C57BL,
pubmed-meshheading:1120935-Organophosphorus Compounds,
pubmed-meshheading:1120935-Ornithine Carbamoyltransferase
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pubmed:year |
1975
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pubmed:articleTitle |
Enzymatic determination of carbamyl phosphate in blood.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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