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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
This study examined mechanisms of Cl- transport in rat lymphocytes under a variety of conditions. Basal intracellular Cl- concentration ([Cl-]i) was not different between cells assayed in the presence of HCO3- or its absence (HEPES). Removal of external Cl- resulted in a fall in [Cl-]i and a rapid rise in intracellular pH (pH(i)). Both Cl- efflux and the rise in pH(i) were blocked by DIDS or removal of external Na+ but were unaffected by furosemide. The mechanisms governing Cl- influx were assessed in cells that had been Cl- depleted for 1 h. Reexposure to Cl- resulted in a rapid rise in [Cl-]i that was partially inhibited by pretreatment with DIDS (57%) and partially inhibited by pretreatment with furosemide (45%). Pretreatment with both compounds together completely blocked Cl- influx. Cl- depletion caused a marked increase in pH(i) that rapidly declined toward normal when the cells were reexposed to Cl-. Preincubation with DIDS completely blocked this decrease in pH(i). In contrast, neither removal of Na+ nor preincubation with furosemide affected the decline in pH(i) when the cells were reexposed to Cl-. We conclude that, in thymic lymphocytes, Cl-/HCO3- (or Cl-/base exchange) regulates both Cl- influx and efflux. Cl- efflux is totally inhibited by DIDS and is mediated by a Na+-dependent Cl-/HCO3- exchanger. Cl- influx is partially DIDS sensitive and partially furosemide sensitive and is mediated by both a Na+-independent Cl-/HCO3- exchanger and by a Na+-K+-2Cl- cotransporter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',7'-bis(carboxyethyl)-5(6)-carboxy...,
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride-Bicarbonate Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/N-(ethoxycarbonylmethyl)-6-methoxyqu...,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolinium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Chloride Symporters
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1931-857X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F314-24
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pubmed:dateRevised |
2011-4-28
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pubmed:meshHeading |
pubmed-meshheading:11208607-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:11208607-Animals,
pubmed-meshheading:11208607-Antiporters,
pubmed-meshheading:11208607-Carrier Proteins,
pubmed-meshheading:11208607-Chloride-Bicarbonate Antiporters,
pubmed-meshheading:11208607-Chlorides,
pubmed-meshheading:11208607-Fluoresceins,
pubmed-meshheading:11208607-Fluorescent Dyes,
pubmed-meshheading:11208607-Hydrogen-Ion Concentration,
pubmed-meshheading:11208607-Quinolinium Compounds,
pubmed-meshheading:11208607-Rats,
pubmed-meshheading:11208607-Rats, Sprague-Dawley,
pubmed-meshheading:11208607-Sodium Chloride,
pubmed-meshheading:11208607-Sodium-Potassium-Chloride Symporters,
pubmed-meshheading:11208607-T-Lymphocytes
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pubmed:year |
2001
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pubmed:articleTitle |
Mechanisms of chloride transport in thymic lymphocytes.
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pubmed:affiliation |
Division of Nephrology and Hypertension, Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA.
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pubmed:publicationType |
Journal Article
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