Source:http://linkedlifedata.com/resource/pubmed/id/11208111
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2001-3-6
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| pubmed:abstractText |
In order to determine if the sequence patterns known to specify internalization represent the majority of possible internalization signals, we identified random sequences capable of causing a reporter protein to be internalized at least several-fold faster than the rate of non-selective internalization of membrane by clathrin-coated pits. A library of influenza hemagglutinin (HA) proteins, bearing short random sequences in place of the wild-type cytoplasmic domain, was prepared in recombinant SV40 virus. The library was expressed and screened for HAs that could internalize anti-HA antibody from the medium. The cytoplasmic sequences of the selected proteins were determined. From a small sample of sequences we detected several that did not resemble those previously identified. The known internalization signals must represent only a subset of the sequences that can serve as internalization signals.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1398-9219
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
282-90
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11208111-Amino Acid Motifs,
pubmed-meshheading:11208111-Amino Acid Sequence,
pubmed-meshheading:11208111-Amino Acid Substitution,
pubmed-meshheading:11208111-Animals,
pubmed-meshheading:11208111-Cell Line,
pubmed-meshheading:11208111-Cercopithecus aethiops,
pubmed-meshheading:11208111-Clathrin-Coated Vesicles,
pubmed-meshheading:11208111-Codon,
pubmed-meshheading:11208111-Endocytosis,
pubmed-meshheading:11208111-Endosomes,
pubmed-meshheading:11208111-Fibroblasts,
pubmed-meshheading:11208111-Gene Library,
pubmed-meshheading:11208111-Genes, Reporter,
pubmed-meshheading:11208111-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:11208111-Influenza A virus,
pubmed-meshheading:11208111-Molecular Sequence Data,
pubmed-meshheading:11208111-Mutagenesis, Site-Directed,
pubmed-meshheading:11208111-Protein Structure, Tertiary,
pubmed-meshheading:11208111-Protein Transport,
pubmed-meshheading:11208111-Random Allocation,
pubmed-meshheading:11208111-Recombinant Fusion Proteins
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| pubmed:year |
2000
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| pubmed:articleTitle |
A screen of random sequences for those that alter the trafficking of the influenza virus hemagglutinin in vivo.
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| pubmed:affiliation |
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235-9038, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|