Source:http://linkedlifedata.com/resource/pubmed/id/11207773
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
A fluorescence in situ hybridization (FISH) protocol suitable for the identification of prokaryotes inhabiting hypersaline environments was developed and applied to several crystallizer ponds with salinities above 36% from a multipond solar saltern in Alicante, Spain. Two morphotypes were abundant in these environments: rods and square or square-like prokaryotes that could be affiliated to Bacteria and Archaea, respectively, by FISH with domain-specific probes. FISH with a newly designed probe proved that the archaeal 16S rDNA sequence most frequently recovered from the crystallizers, SPhT, originated from the dominant square-like prokaryotes. These uncultured prokaryotes have the morphology of Walsby's square bacteria. Additionally, FISH with a probe targeted to the genus Haloarcula, members of which are frequently isolated from this environment, indicated that this genus accounts for less than 0.1% of the total prokaryotic community.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Archaeal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1462-2912
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
517-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11207773-Archaea,
pubmed-meshheading:11207773-DNA, Archaeal,
pubmed-meshheading:11207773-DNA, Ribosomal,
pubmed-meshheading:11207773-DNA Probes,
pubmed-meshheading:11207773-Haloarcula,
pubmed-meshheading:11207773-Haloferax,
pubmed-meshheading:11207773-In Situ Hybridization, Fluorescence,
pubmed-meshheading:11207773-RNA, Ribosomal, 16S,
pubmed-meshheading:11207773-Sodium Chloride,
pubmed-meshheading:11207773-Water Microbiology
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pubmed:year |
1999
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pubmed:articleTitle |
Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds.
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pubmed:affiliation |
Departamento de Biotecnología, Universidad de Alicante, San Vicente del Raspeig, Spain. anton@ua.es
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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