Linked Life Data

11206779

Source:http://linkedlifedata.com/resource/pubmed/id/11206779

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-2-6
pubmed:abstractText
A novel bi-column method for purifying transcription factors, using two different columns and two different elution strategies is described. Lac repressor elutes at lower heparin concentrations from a lower affinity lactose operatorl (Op1)-Sepharose column than from a higher affinity column containing the same sequence with a T18:A18 tail (Op1T18). A bi-column method was developed in which lac repressor fusion protein is eluted from the Op1-Sepharose with a low heparin concentration and trapped on a Op1T18-Sepharose column because of its higher affinity for the lac repressor protein. Elution of the latter column with buffer containing a high salt concentration gives significantly purer transcription factor than the conventionally used single column methods and removes residual heparin. Highly pure CAAT enhancer binding protein and the B3 transcription factor are also obtained by using variants of this bi-column method.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
905
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-9
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Bi-column method for purification of transcription factors.
pubmed:affiliation
Department of Biochemistry, University of Tennessee, Memphis 38163, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.